CUGBP1/CELF1 Antibody (3B1)


Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.
Immunocytochemistry/ Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica more
Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 more
Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Pm, RbSpecies Glossary
Applications WB, Simple Western, Flow, GS, ICC/IF, IHC, IP
1 mg/ml

Order Details

CUGBP1/CELF1 Antibody (3B1) Summary

Full-length human CUGBP1 [UniProt# Q92879]
IgG1 Kappa
Protein G purified
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  • Flow Cytometry 1 ug per million cells
  • Gel Super Shift Assays reported in scientific literature
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation reported in scientific literature
  • Simple Western 1:200
  • Western Blot 1:500
Application Notes
In Western Blot, a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
CUGBP1/CELF1 Overexpression Lysate
Read Publications using
NB200-316 in the following applications:

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.02% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for CUGBP1/CELF1 Antibody (3B1)

  • BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
  • bruno-like 2
  • Bruno-like protein 2
  • CELF1
  • CUG RNA-binding protein
  • CUG triplet repeat, RNA binding protein 1
  • CUG triplet repeat, RNA-binding protein 1
  • CUG-BP- and ETR-3-like factor 1
  • CUGBP Elav-like family member 1
  • CUG-BP
  • CUGBP, Elav-like family member 1
  • CUGBP1
  • CUG-BP1
  • CUGBP1CUG triplet repeat RNA-binding protein 1
  • D2Wsu101e
  • Deadenylation factor CUG-BP
  • EDEN-BP homolog
  • embryo deadenylation element binding protein
  • Embryo deadenylation element-binding protein homolog
  • HNAB50
  • NAB50
  • NAB50CUG-BP1
  • nuclear polyadenylated RNA-binding protein, 50-kD
  • RNA-binding protein BRUNOL-2


CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CUGBP1/CELF1 Antibody (NB200-316)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 7 applications: EMSA, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB200-316 Applications Species
Markmiller S, Sathe S, Server KL et al. Persistent mRNA localization defects and cell death in ALS neurons caused by transient cellular stress Cell reports 2021-09-07 [PMID: 34496257]
de Haro M, Al-Ramahi I, Jones KR et al. Smaug/SAMD4A Restores Translational Activity of CUGBP1 and Suppresses CUG-Induced Myopathy. PLoS Genet 2013-04-01 [PMID: 23637619] (IP, WB, ICC/IF, Primate, Human) IP, WB, ICC/IF Primate, Human
Xiao Q, Ford AL, Xu J et al. Bcl-x Pre-mRNA Splicing Regulates Brain Injury after Neonatal Hypoxia-Ischemia J Neurosci 2012-09-26 [PMID: 23015448] (IF/IHC, ICC/IF, WB, EMSA, Mouse, Rat) IF/IHC, ICC/IF, WB, EMSA Mouse, Rat
Ward AJ, Rimer M, Killian JM, Dowling JJ, Cooper TA. CUGBP1 overexpression in mouse skeletal muscle reproduces features of myotonic dystrophy type 1. Hum Mol Genet;19(18):3614-22. 2010-09-15 [PMID: 20603324]
Hashem V, Galloway JN, Mori M, Willemsen R, Oostra BA, Paylor R, Nelson DL. Ectopic expression of CGG containing mRNA is neurotoxic in mammals. Hum Mol Genet;18(13):2443-51. 2009-07-01 [PMID: 19377084] (IHC-P, Mouse) IHC-P Mouse
Orengo JP, Chambon P, Metzger D, Mosier DR, Snipes GJ, Cooper TA. Expanded CTG repeats within the DMPK 3' UTR causes severe skeletal muscle wasting in an inducible mouse model for myotonic dystrophy. Proc Natl Acad Sci U S A;105(7):2646-51. 2008-02-19 [PMID: 18272483]
Kress, C et al. Inactivation of CUG-BP1/CELF1 causes growth, viability, and spermatogenesis defects in mice. Mol Cell Biol;27(3):1146-57. 2007-02-01 [PMID: 17130239] (WB, Mouse) WB Mouse
Lin X, Miller JW, Mankodi A, Kanadia RN, Yuan Y, Moxley RT, Swanson MS, Thornton CA. Failure of MBNL1-dependent post-natal splicing transitions in myotonic dystrophy. Hum Mol Genet;15(13):2087-97. 2006-07-01 [PMID: 16717059] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Mankodi A, Lin X, Blaxall BC, Swanson MS, Thornton CA. Nuclear RNA foci in the heart in myotonic dystrophy. Circ Res;97(11):1152-5. 2005-11-25 [PMID: 16254211] (IHC-Fr, Human) IHC-Fr Human
Ami Mankodi, MD, Patana Teng-Umnuay, MD, PhD, Matt Krym, BSc, Don Henderson, BSc, Maurice Swanson, PhD, Charles A Thornton, MD. Ribonuclear Inclusions in Skeletal Muscle in Myotonic Dystrophy Types 1 2. Ann Neurol; 54:760. 2003-01-01 [PMID: 14681885] (ICC/IF, Human) ICC/IF Human
Show All 12 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CUGBP1/CELF1 Antibody (NB200-316). (Showing 1 - 1 of 1 FAQs).

  1. We have a question about one of your products: Has the epitope been mapped for this antibody (NB 200-316)? Thanks for any additional information you may be able to provide.
    • We have not epitope mapped this antibody, so no further information is available.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol CELF1