Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.
Immunocytochemistry/ Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica ...read more
Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Hu, Mu, Rt, Po, Bv, Pm, RbSpecies Glossary
WB, Simple Western, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
This CUGBP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections and Western Blot where a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF. Immunoprecipitation and Gel Super Shift Assays were reported in scientific literature.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.02% Sodium Azide
Protein G purified
Alternate Names for CUGBP1/CELF1 Antibody (3B1)
BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
Bruno-like protein 2
CUG RNA-binding protein
CUG triplet repeat, RNA binding protein 1
CUG triplet repeat, RNA-binding protein 1
CUG-BP- and ETR-3-like factor 1
CUGBP Elav-like family member 1
CUGBP, Elav-like family member 1
CUGBP1CUG triplet repeat RNA-binding protein 1
Deadenylation factor CUG-BP
embryo deadenylation element binding protein
Embryo deadenylation element-binding protein homolog
nuclear polyadenylated RNA-binding protein, 50-kD
RNA-binding protein BRUNOL-2
CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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