CUGBP1/CELF1 Antibody (3B1)

Images

 
Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.
Immunocytochemistry/ Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica ...read more
Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, RbSpecies Glossary
Applications WB, Simple Western, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
3B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CUGBP1/CELF1 Antibody (3B1) Summary

Immunogen
Full-length human CUGBP1 [UniProt# Q92879]
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CELF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:200
  • Flow Cytometry 1 ug per million cells
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
Application Notes
This CUGBP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen and paraffin embedded sections and Western Blot where a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF. Immunoprecipitation and Gel Super Shift Assays were reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CUGBP1/CELF1 Overexpression Lysate
CUGBP1/CELF1 Knockout 293T Cell Lysate
Publications
Read Publications using
NB200-316 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CUGBP1/CELF1 Antibody (3B1)

  • BRUNOL2
  • BRUNOL250 kDa nuclear polyadenylated RNA-binding protein
  • bruno-like 2
  • Bruno-like protein 2
  • CELF1
  • CUG RNA-binding protein
  • CUG triplet repeat, RNA binding protein 1
  • CUG triplet repeat, RNA-binding protein 1
  • CUG-BP- and ETR-3-like factor 1
  • CUGBP Elav-like family member 1
  • CUG-BP
  • CUGBP, Elav-like family member 1
  • CUGBP1
  • CUG-BP1
  • CUGBP1CUG triplet repeat RNA-binding protein 1
  • CUGBPCELF-1
  • D2Wsu101e
  • Deadenylation factor CUG-BP
  • EDEN-BP homolog
  • EDEN-BP
  • embryo deadenylation element binding protein
  • Embryo deadenylation element-binding protein homolog
  • HNAB50
  • NAB50
  • NAB50CUG-BP1
  • NAPOR
  • nuclear polyadenylated RNA-binding protein, 50-kD
  • RNA-binding protein BRUNOL-2

Background

CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB6967
Species: Hu
Applications: WB
NBP2-16035
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00004154-M02
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
NB110-85519
Species: Hu, Mu
Applications: WB, ELISA, IHC, IP, KD
NBP2-42632
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-89932
Species: Hu
Applications: IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-12793
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP1-89933
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00023049-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-31308
Species: Hu, Mu, Rt, Bv, Fe
Applications: WB, ICC/IF, IHC, IHC-P
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-45731
Species: All-NA
Applications: WB, IHC, IHC-P
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, PA, B/N, KD
MAB6570
Species: Hu
Applications: WB, IHC
NBP2-67635
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP
NB200-316
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb
Applications: WB, Simple Western, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for CUGBP1/CELF1 Antibody (NB200-316)(11)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 7 applications: EMSA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
EMSA
(1)
ICC/IF
(4)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(4)
Rat
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB200-316 Applications Species
de Haro M, Al-Ramahi I, Jones KR et al. Smaug/SAMD4A Restores Translational Activity of CUGBP1 and Suppresses CUG-Induced Myopathy. PLoS Genet 2013 Apr [PMID: 23637619] (IP, ICC/IF, WB, Primate, Human) IP, ICC/IF, WB Primate, Human
Xiao Q, Ford AL, Xu J et al. Bcl-x Pre-mRNA Splicing Regulates Brain Injury after Neonatal Hypoxia-Ischemia J Neurosci 2012 Sep 26 [PMID: 23015448] (WB, EMSA, ICC/IF, IHC, Rat, Mouse) WB, EMSA, ICC/IF, IHC Rat, Mouse
Ward AJ, Rimer M, Killian JM, Dowling JJ, Cooper TA. CUGBP1 overexpression in mouse skeletal muscle reproduces features of myotonic dystrophy type 1. Hum Mol Genet;19(18):3614-22. 2010 Sep 15. [PMID: 20603324]
Hashem V, Galloway JN, Mori M, Willemsen R, Oostra BA, Paylor R, Nelson DL. Ectopic expression of CGG containing mRNA is neurotoxic in mammals. Hum Mol Genet;18(13):2443-51. 2009 Jul 1. [PMID: 19377084] (IHC-P, Mouse) IHC-P Mouse
Orengo JP, Chambon P, Metzger D, Mosier DR, Snipes GJ, Cooper TA. Expanded CTG repeats within the DMPK 3' UTR causes severe skeletal muscle wasting in an inducible mouse model for myotonic dystrophy. Proc Natl Acad Sci U S A;105(7):2646-51. 2008 Feb 19. [PMID: 18272483]
Kress, C et al. Inactivation of CUG-BP1/CELF1 causes growth, viability, and spermatogenesis defects in mice. Mol Cell Biol;27(3):1146-57. 2007 Feb. [PMID: 17130239] (WB, Mouse) WB Mouse
Lin X, Miller JW, Mankodi A, Kanadia RN, Yuan Y, Moxley RT, Swanson MS, Thornton CA. Failure of MBNL1-dependent post-natal splicing transitions in myotonic dystrophy. Hum Mol Genet;15(13):2087-97. 2006 Jul 1. [PMID: 16717059] (WB, ICC/IF, Human, Mouse) WB, ICC/IF Human, Mouse
Mankodi A, Lin X, Blaxall BC, Swanson MS, Thornton CA. Nuclear RNA foci in the heart in myotonic dystrophy. Circ Res;97(11):1152-5. 2005 Nov 25. [PMID: 16254211] (IHC-Fr, Human) IHC-Fr Human
Mankodi A, Teng-Umnuay P, Krym M, Henderson D, Swanson M, Thornton CA. Ribonuclear inclusions in skeletal muscle in myotonic dystrophy types 1 and 2. Ann Neurol;54(6):760-8. 2003 Dec. [PMID: 14681885] (ICC/IF, Human) ICC/IF Human
Savkur RS, Philips AV, Cooper TA. Aberrant regulation of insulin receptor alternative splicing is associated with insulin resistance in myotonic dystrophy. Nat Genet;29(1):40-7. 2001 Sep. [PMID: 11528389] (WB, Human) WB Human
Show All 11 Publications.

Reviews for CUGBP1/CELF1 Antibody (NB200-316) (0)

There are no reviews for CUGBP1/CELF1 Antibody (NB200-316). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for CUGBP1/CELF1 Antibody (NB200-316): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CUGBP1/CELF1 Antibody (NB200-316). (Showing 1 - 1 of 1 FAQs).

  1. We have a question about one of your products: Has the epitope been mapped for this antibody (NB 200-316)? Thanks for any additional information you may be able to provide.
    • We have not epitope mapped this antibody, so no further information is available.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-316AF350
Alexa Fluor 405 NB200-316AF405
Alexa Fluor 488 NB200-316AF488
Alexa Fluor 532 NB200-316AF532
Alexa Fluor 594 NB200-316AF594
Alexa Fluor 647 NB200-316AF647
Alexa Fluor 700 NB200-316AF700
Alexa Fluor 750 NB200-316AF750
Allophycocyanin NB200-316APC
Biotin NB200-316B
DyLight 350 NB200-316UV
DyLight 405 NB200-316V
DyLight 488 NB200-316G
DyLight 550 NB200-316R
DyLight 594 NB200-316DL594
DyLight 650 NB200-316C
DyLight 680 NB200-316FR
DyLight 755 NB200-316IR
FITC NB200-316F
HRP NB200-316H
Janelia Fluor 549 NB200-316JF549
Janelia Fluor 646 NB200-316JF646
PE NB200-316PE

Additional CUGBP1/CELF1 Products

Bioinformatics Tool for CUGBP1/CELF1 Antibody (NB200-316)

Discover related pathways, diseases and genes to CUGBP1/CELF1 Antibody (NB200-316). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CUGBP1/CELF1 Antibody (NB200-316)

Discover more about diseases related to CUGBP1/CELF1 Antibody (NB200-316).
 

Pathways for CUGBP1/CELF1 Antibody (NB200-316)

View related products by pathway.

PTMs for CUGBP1/CELF1 Antibody (NB200-316)

Learn more about PTMs related to CUGBP1/CELF1 Antibody (NB200-316).
 

Research Areas for CUGBP1/CELF1 Antibody (NB200-316)

Find related products by research area.

Blogs on CUGBP1/CELF1

There are no specific blogs for CUGBP1/CELF1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CUGBP1/CELF1 Antibody (3B1) and receive a gift card or discount.

Bioinformatics

Gene Symbol CELF1
Entrez
Uniprot