DLL4 Antibody

Images

 
Western Blot: DLL4 Antibody [NB600-892] - Shows detection of a 74-kDa band corresponding to Delta-4 in a lysate prepared from mouse pancreatic tissue. Approximately 20 ug of lysate was run on SDS-PAGE and transferred ...read more
Immunohistochemistry-Paraffin: DLL4 Antibody [NB600-892] - This antibody was used at 20ug/mL in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DLL4 Antibody Summary

Immunogen
Anti-DLL4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DLL4.
Localization
Type I membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLL4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2500
  • ELISA 1:10000 - 1:50000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 ug/ml-20 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml-20 ug/ml
Application Notes
Expect a band approximately 74 kDa in size corresponding - DLL4 by western blotting in the appropriate cell lysate or extract.Flow Cytometry was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28352669). Use in FLOW reported in scientific literature (PMID: 19915058).
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 1
using
NB600-892 in the following applications:

Publications
Read Publications using
NB600-892 in the following applications:

Reactivity Notes

Chimpanzee 91%. Mouse reactivity reported in scientific literature (PMID: 27735989). Rat reactivity reported in scientific literature (PMID: 19828677).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DLL4 Antibody

  • Delta 4 precursor
  • delta 4
  • delta ligand 4
  • delta4
  • delta-like 4 (Drosophila)
  • delta-like 4 homolog (Drosophila)
  • delta-like 4 homolog
  • delta-like 4 protein
  • delta-like protein 4
  • DLL4
  • Drosophila Delta homolog 4
  • hdelta2
  • MGC126344
  • notch ligand delta-2
  • notch ligand DLL4

Background

DLL4 (delta-like ligand 4) is a key player of Notch signaling pathway that has been extensively studied in tumor angiogenesis wherein its blockade results in non-productive angiogenesis followed by halted tumor growth. Notch signaling is evolutionarily conserved, and is consists of four receptors (Notch1-4) and five transmembrane ligands (Jagged1, 2, Delta like ligands 1, 3, 4). Among Notch ligands, DLL4 activates Notch1/Notch4 for serving important role in normal development and tissue homeostasis by implicating in immune system, gastro-intestinal tract, and vascular development in embryonic/postnatal development. DLL4 is largely an arterial-endothelial-specific gene expressed during embryonic development, while in adults its expression is mostly restricted to small arteries and capillary networks. Haploinsufficiency of DLL4 in mice results in embryonic lethality due to abnormal vasculogenesis characterized by a dense, disrupted and non-functional capillary network. DLL4 has a negative feedback effect on VEGFR2 signaling and in tumor vasculature, it plays a role in negative regulation of tumor vascularization. DLL4 protein shows cytoplasmic expression in cancer cells from where it translocates to nuclei upon hypoxic stimulus and DLL4 induced Notch activation is intimately related to tumor invasion as well as tumor-initiating cell frequency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DLL4 Antibody (NB600-892)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(1)
ICC/IF
(2)
IHC
(3)
IHC-P
(1)
WB
(3)
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Human
(6)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB600-892 Applications Species
Shao, L;Sottoriva, K;Palasiewicz, K;Zhang, J;Hyun, J;Soni, SS;Paik, NY;Gao, X;Cuervo, H;Malik, AB;Rehman, J;Lucas, D;Pajcini, KV; A Tie2-Notch1 signaling axis regulates regeneration of the endothelial bone marrow niche Haematologica Mar 28 2019 [PMID: 30923091] (WB, Mouse) WB Mouse
Zheng X, Narayanan S, Sunkari VG et al. Triggering of a Dll4-Notch1 loop impairs wound healing in diabetes Proc. Natl. Acad. Sci. U.S.A. Apr 2 2019 [PMID: 30886104] (ICC/IF, Mouse) ICC/IF Mouse
Davis RB, Kechele DO, Blakeney ES et al. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation. JCI Insight. Mar 23 2017 [PMID: 28352669] (ICC/IF, Human) ICC/IF Human
Kontomanolis E, Mitrakas A, Giatromanolaki A et al. A pilot study on plasma levels of micro-RNAs involved in angiogenesis and vascular maturation in patients with breast cancer Med. Oncol Feb 1 2017 [PMID: 28058628] (IHC, Human) IHC Human
Muguruma Yukari, Hozumi Katsuto, Warita Hiroyuki et al. Maintenance of Bone Homeostasis by DLL1-Mediated Notch Signaling. J Cell Physiol 2017 [PMID: 27735989] (IHC, Mouse) IHC Mouse
Kontomanolis E, Panteliadou M, Giatromanolaki A et al. Delta-like ligand 4 (DLL4) in the plasma and neoplastic tissues from breast cancer patients: correlation with metastasis. Med. Oncol. 2014 May 01 [PMID: 24696220] (IHC-P, Human) IHC-P Human
Anjos-Afonso F, Currie E, Palmer HG et al. CD34(-) cells at the apex of the human hematopoietic stem cell hierarchy have distinctive cellular and molecular signatures. Cell Stem Cell. 2013 Aug 1 [PMID: 23910083] (IHC, Human) IHC Human
Wu S, Esumi S, Watanabe K et al. Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon. Development 2011 Jun [PMID: 21561989]
Lipscomb MW, Chen L, Taylor JL et al. Ectopic T-bet expression licenses dendritic cells for IL-12-independent priming of type 1 T cells in vitro. J Immunol;183(11):7250-8. 2009 Dec 1. [PMID: 19915058] (FLOW, Human) FLOW Human
Gupta S, Li S, Abedin MJ et al. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol :00451. 2009 [PMID: 19828677] (WB, Rat) WB Rat
Show All 16 Publications.

Review for DLL4 Antibody (NB600-892) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-892:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot DLL4 NB600-892
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1
reviewed by:
Manjinder Cheema
WB Mouse 02/24/2020
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Summary

ApplicationWestern Blot
Sample TestedAdult kidney,Liver,Mouse Spleen,Brain
SpeciesMouse
Lot38811

Comments

CommentsLot of non-specific bands

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DLL4 Antibody (NB600-892). (Showing 1 - 1 of 1 FAQs).

  1. Is the antibody intended to work on paraffin sections only? Did you test it on frozen sections?
    • In our QC data, I found that we have successfully tested this antibody for Western Blot and IHC-P. We have not confirmed the antibody for the IHC-Fr application; however, I would not discourage you from trying it on your frozen sections. For IHC-P sections this antibody worked well at concentrations from 10 ug/ml - 20 ug/ml and you may try this antibody for IHC on your frozen sections considering similar dilutions as a starting point. Moreover, considering your case, we can recommend our Innovators Reward Program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the Innovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for DLL4 Antibody (NB600-892)

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Blogs on DLL4.

New DLL4 Vaccine May Prevent Breast Tumors
We at Novus Biologicals have a large antibody database devoted to signalling pathways. These underpin every area of molecular biological research, including cancer. Among our cell signalling antibodies is one targeted to DLL4 (Delta-like protein 4). D...  Read full blog post.

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Recent Reviews

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Manjinder Cheema
02/24/2020
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol DLL4
Entrez
Uniprot