Recombinant Human Dkk-1 Protein

Product Discontinued

Recombinant Human Dkk-1 Protein Summary

• Description: A recombinant protein to Dkk-1.
  
  

  Source: Spodoptera frugiperda, Sf 21 (baculovirus)
  Predicted molecular mass of approximately 25.8 kDa, a single polypeptide chain containing 235 amino acids.
  Aminio Acid Sequence: Thr32 - His266

• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in PBS, pH 7.2.
• Details of Functionality: The ED50, as determined by inhibition of Wnt-3a induced alkaline phosphatase production using mouse MC3T3E1 cells, is less than 150 ng/ml.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: DKK1
• Endotoxin Note: Less than 1 EU/ug of the rHuDkk-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with sterilized PBS or aqueous buffer to a final concentration of 0.1 mg/ml.

Alternate Names for Recombinant Human Dkk-1 Protein

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1), along with Dkk -2, -3, -4, and Soggy, are secreted proteins with two conserved cysteine-rich domains. Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86% aa sequence identity with mouse and rat Dkk-1. Dkk-1 antagonizes Wnt signaling by forming ternary complexes with LRP5/6 and Kremen1 or Kremen2. Its function is important during the development of limbs, neurons, hair follicles, retina, and bone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DKK1