Dishevelled-3 Antibody - Azide and BSA Free

Western Blot: Dishevelled-3 Antibody [NBP3-03068] - Western blot analysis of extracts of various cell lines, using Dishevelled-3 antibody (NBP3-03068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat ovary using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse kidney using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Dishevelled-3 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Dishevelled-3 Antibody - Azide and BSA Free (NBP3-03068) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-650 of human Dishevelled-3 (NP_004414.3). RSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DVL3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Dishevelled-3 Antibody - Azide and BSA Free

• dishevelled 3 (homologous to Drosophila dsh)
• dishevelled, dsh homolog 3 (Drosophila)
• Dishevelled3
• Dishevelled-3
• DSH homolog 3
• DVL3
• KIAA0208dishevelled-3
• segment polarity protein dishevelled homolog DVL-3

Background

Dishevelled 3 is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DVL3