Dishevelled-2 Antibody

Images

 
Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Whole cell extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody Internal diluted at 1:500. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388] - Paraffin-embedded lung SCC, using antibody at 1:100 dilution.
Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Sample(30 ug whole cell lysate)A: MOLT4 )B: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.04 mg/ml

Order Details

Dishevelled-2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (99%), Bovine (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DVL2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (80%), Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.04 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dishevelled-2 Antibody

  • dishevelled 2 (homologous to Drosophila dsh)
  • dishevelled, dsh homolog 2 (Drosophila)
  • Dishevelled2
  • Dishevelled-2
  • DSH homolog 2
  • DVL2
  • segment polarity protein dishevelled homolog DVL-2

Background

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DVL2
Entrez