Dishevelled-2 Antibody

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Intracellular distribution of Wwox (dilution 1:1000, AF6399), Dvl-1 (dilution 1:700, NBP1-58318), Dvl-2 (Dishevelled-2, dilution 1:1000,) and Dvl-3 (dilution 1:200, NBP2-24674) proteins in HET-1A and SCC-15 cell lines. Image collected and cropped by CiteAb from the following publication (www.jcancer.org/v11p4030.htm) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388] - Paraffin-embedded lung SCC, using antibody at 1:100 dilution.

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Sample(30 ug whole cell lysate)A: MOLT4 )B: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Whole cell extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dishevelled-2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (99%), Rhesus Macaque (99%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DVL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (80%), Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Dishevelled-2 Antibody

• dishevelled 2 (homologous to Drosophila dsh)
• dishevelled, dsh homolog 2 (Drosophila)
• Dishevelled2
• Dishevelled-2
• DSH homolog 2
• DVL2
• segment polarity protein dishevelled homolog DVL-2

Background

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Dishevelled-2 Antibody (NBP1-32388)(1)

Publication using NBP1-32388

• Celebi A, Orhan C, et al. Silencing of Wwox Increases Nuclear Import of Dvl proteins in Head and Neck Cancer. J Cancer 2020-05-06 [PMID: 32368285] (WB, Human)

Bioinformatics

• Gene Symbol: DVL2