DHX58 Antibody

Western Blot: DHX58 Antibody [NBP1-20939] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Immunohistochemistry-Paraffin: DHX58 Antibody [NBP1-20939] - Staining of paraffin embedded Human Cortex. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DHX58 Antibody Summary

• Description: Novus Biologicals Goat DHX58 Antibody (NBP1-20939) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DHX58 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence DFLQHCAENLSD corresponding to C-Terminus according to NP_077024.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DHX58
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 8 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 80 kDa band observed in human liver lysates (calculated MW of 76.6 kDa band according to NP_077024.2). An additional band of unknown identity was also consistently observed at 24 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX58 Antibody

• D11lgp2e
• D11LGP2probable ATP-dependent RNA helicase DHX58
• DEXH (Asp-Glu-X-His) box polypeptide 58
• EC 3.6.4.13
• LGP2ortholog of mouse D11lgp2
• Probable ATP-dependent helicase LGP2
• Protein D11Lgp2 homolog
• RIG-I-like receptor LGP2
• RLR
• RNA helicase LGP2

Background

Negative regulator of host innate immune defense against viruses. The repressor domain of DHX58 interactswith DDX58 and negatively regulates DDX58-mediated signaling. Binds dsRNA produced during viral replication, inparticuliar HCV RNA. Binding occurs at blunt dsRNA termini

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DHX58 Antibody (NBP1-20939)(1)

Publication using NBP1-20939

• Saito T, Hirai R, Loo YM et al. Regulation of innate antiviral defenses through a shared repressor domain in RIG-I and LGP2. Proc Natl Acad Sci U S A 2007-01-09 [PMID: 17190814]

Bioinformatics

• Gene Symbol: DHX58
• Entrez:
  • 79132(Human)
• Uniprot:
  • NP_077024.2(Human)