DGKH Antibody - BSA Free

Immunoprecipitation: DGKH Antibody - BSA Free [NBP3-48019] - IP Result of anti-DGKH (IP:, 5ug; Detection: 1:500) with mouse brain tissue lysate 2640ug.

Immunohistochemistry: DGKH Antibody - BSA Free [NBP3-48019] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (DGKH Antibody) at dilution of 1:200

Western Blot: DGKH Antibody - BSA Free [NBP3-48019] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (DGKH antibody) at dilution of 1:300

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DGKH Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DGKH Antibody - BSA Free (NBP3-48019) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: diacylglycerol kinase, eta. (Uniprot# Q86XP1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGKH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:50-1:500
  • Immunoprecipitation 1:200-1:2000
  • Western Blot 1:500-1:2000
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DGKH Antibody - BSA Free

• DAG kinase eta
• DGKeta
• DGK-eta
• diacylglycerol kinase eta
• diacylglycerol kinase, eta
• Diglyceride kinase eta
• DKFZp761I1510
• EC 2.7.1.107

Background

DGKH, also known as Diacylglycerol kinase eta, consists of four isoforms of sizes 134.9 kDa, 128 kDa, 122.4 kDa, and 120.6 kDa and is involved in activating the signaling pathway Ras/B-Raf/C-Raf/MEK/ERK in order to promote cell growth. Current research is exploring the effect of the protein on diseases and disorders such as measles, neuronitis, bipolar disorder, and schizophrenia. The protein interacts with AGPAT2, AGPAT4, CHPT1, ARRB1, and DGKD proteins while in hemostasis, signal transduction, and metabolic pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKH