DGK-delta Antibody (625423)

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Western blot shows lysates of TT human medullary thyroid cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human DGK‑δ Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
625423
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

DGK-delta Antibody (625423) Summary

Immunogen
E. coli-derived recombinant human DGF-δ
Ile570-Thr717
Accession # Q16760
Specificity
Detects human DGF-δ in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DGKD
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-delta Antibody (625423)

  • 130 kDa diacylglycerol kinase
  • DAG kinase delta
  • DGKD
  • dgkd-2
  • DGKdelta
  • DGK-delta
  • diacylglycerol kinase delta
  • diacylglycerol kinase, delta (130kD)
  • diacylglycerol kinase, delta 130kDa
  • Diglyceride kinase delta
  • FLJ26930
  • KIAA0145EC 2.7.1.107

Background

Diacylglycerol kinase delta (DGK-δ) is a widely expressed 140 kDa member of the DGK family of kinases that phosphorylate the lipid diacylglycerol. DGK-δ contains a pleckstrin homology domain (aa 53-146), two zinc finger domains (aa 163-213 and aa 235-286), a catalytic domain (aa 317-451), and a sterile alpha motif (aa 1145‑1208). An isoform of DGK-δ with a shortened N-terminus is expressed predominantly in ovary and spleen. The two isoforms can form intracellular homooligomers or heterooligomers with each other. The oligomers are disrupted by PKC following phorbol ester stimulation. DGK-δ regulates PKC mediated signaling downstream of the EGF receptor. Within aa 570-717, human DGK-δ shares 82% aa sequence identity with mouse and rat DGK-δ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DGKD
Entrez
Uniprot