DGK-theta Antibody - BSA Free

Immunohistochemistry-Paraffin: DGK-theta Antibody [NBP1-85317] - Staining of human stomach shows strong cytoplasmic positivity in superficial glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DGK-theta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DGK-theta Antibody - BSA Free (NBP1-85317) is a polyclonal antibody validated for use in IHC and WB. Anti-DGK-theta Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGKQ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 24369117). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  DGK-theta Protein (NBP1-85317PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (84%). Human reactivity reported in scientific literature (PMID: 23610160).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DGK-theta Antibody - BSA Free

• DAG kinase theta
• DAGK
• DAGK4EC 2.7.1.107
• DAGK7
• DGKQ
• DGKtheta
• DGK-theta
• diacylglycerol kinase theta
• diacylglycerol kinase, theta (110kD)
• diacylglycerol kinase, theta 110kDa
• Diglyceride kinase theta

Background

Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-theta is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-theta co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP(2)). DGK-theta is the isoform responsive to alpha-Thrombin stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGK-theta Antibody (NBP1-85317)(2)

Publications using NBP1-85317

• Cai K, Lucki NC, Sewer MB. Silencing diacylglycerol kinase-theta expression reduces steroid hormone biosynthesis and cholesterol metabolism in human adrenocortical cells. Biochim Biophys Acta 2014-04-04 [PMID: 24369117] (WB, Human)
• Cai K, Sewer MB. cAMP-stimulated transcription of DGKO requires steroidogenic factor 1 and sterol regulatory element binding protein 1 J Lipid Res 2013-08-01 [PMID: 23610160] (WB, Human)

Bioinformatics

• Gene Symbol: DGKQ