DGAT2 Overexpression Lysate (Native)

Images

 
Western Blot: DGAT2 Overexpression Lysate (Adult Normal) [NBL1-09845] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DGAT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DGAT2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203920 and based on accession number NM_032564. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens diacylglycerol O-acyltransferase homolog 2 (mouse) (DGAT2), mRNA.
Gene
DGAT2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
43.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DGAT2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DGAT2 Overexpression Lysate (Native)

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-41487
Species: Hu, Mu, Rt, Bv, Ze
Applications: WB, ICC/IF
NBP2-49395
Species: Hu
Applications: IHC
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBP2-50444
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF7550
Species: Hu
Applications: WB
NBP1-85691
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NBP2-49154
Species: Hu
Applications: IHC
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-41211
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IF
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-00637
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF6898
Species: Hu, Mu, Rt
Applications: WB
NBL1-09845
Species: Hu
Applications: WB

Publications for DGAT2 Lysate (NBL1-09845) (0)

There are no publications for DGAT2 Lysate (NBL1-09845).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DGAT2 Lysate (NBL1-09845) (0)

There are no reviews for DGAT2 Lysate (NBL1-09845). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DGAT2 Lysate (NBL1-09845) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DGAT2 Products

Bioinformatics Tool for DGAT2 Lysate (NBL1-09845)

Discover related pathways, diseases and genes to DGAT2 Lysate (NBL1-09845). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DGAT2 Lysate (NBL1-09845)

Discover more about diseases related to DGAT2 Lysate (NBL1-09845).
 

Pathways for DGAT2 Lysate (NBL1-09845)

View related products by pathway.

PTMs for DGAT2 Lysate (NBL1-09845)

Learn more about PTMs related to DGAT2 Lysate (NBL1-09845).

Blogs on DGAT2.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

Customers Who Bought This Also Bought

DGAT2 Antibody
NBP1-71701

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DGAT2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DGAT2
Entrez