| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71701SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05769SS | |||
NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="DGAT2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human DGAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7256) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7256-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92727-0.02ml | |||
![Western Blot: DGAT2 Antibody [NBP1-71701] - Analysis of DGAT2 in A. human liver lysate and B. mouse brain lysate.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Western-Blot-NBP1-71701-img0003.jpg "Western Blot: DGAT2 Antibody [NBP1-71701] - Analysis of DGAT2 in A. human liver lysate and B. mouse brain lysate.")
![Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05769-img0005.jpg "Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: DGAT2 Antibody [NBP3-05769] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of DGAT2, catalog number NBP3-05769 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic staing is observed.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunohistochemistry-Paraffin-NBP3-05769-img0001.jpg)
![Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on NIH3T3 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Intracellular-NBP3-05769-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DGAT2 Antibody [NBP3-05769] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-DGAT2 Antibody NBP3-05769 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05769-img0004.jpg)
![Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on HepG2 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Intracellular-NBP3-05769-img0002.jpg)
![Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunohistochemistry-NB100-57851-img0001.jpg "Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: DGAT2 Antibody [NB100-57851] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Cytometry-NB100-57851-img0006.jpg)
CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ![Western Blot: DGAT2 Antibody [NBP2-92727] - Analysis of extracts of various cell lines, using DGAT2 antibody (NBP2-92727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DGAT2 Antibody-Western Blot-NBP2-92727-img0002.jpg "Western Blot: DGAT2 Antibody [NBP2-92727] - Analysis of extracts of various cell lines, using DGAT2 antibody (NBP2-92727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: DGAT2 Antibody [NBP2-92727] - Analysis of extracts of Rat liver cells, using DGAT2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .(Exposure time: 60s).](http://images.novusbio.com/fullsize/DGAT2-Antibody-Western-Blot-NBP2-92727-img0001.jpg)
![Western Blot: DGAT2 Antibody (4C1) [H00084649-M03] - Analysis of DGAT2 expression in MCF-7.](http://images.novusbio.com/fullsize/DGAT2-Antibody-4C1-Western-Blot-H00084649-M03-img0002.jpg "Western Blot: DGAT2 Antibody (4C1) [H00084649-M03] - Analysis of DGAT2 expression in MCF-7.")
![Western Blot: DGAT2 Antibody (4C1) [H00084649-M03] - Analysis of DGAT2 expression in human stomach.](http://images.novusbio.com/fullsize/DGAT2-Antibody-4C1-Western-Blot-H00084649-M03-img0003.jpg)
![ELISA: DGAT2 Antibody (4C1) [H00084649-M03] - Detection limit for recombinant GST tagged DGAT2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DGAT2-Antibody-4C1-Sandwich-ELISA-H00084649-M03-img0001.jpg)
![ELISA: Human DGAT2 ELISA Kit (Colorimetric) [NBP3-31403] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31403_human-dgat2-elisa-kit-colorimetric-206202415301521.png "ELISA: Human DGAT2 ELISA Kit (Colorimetric) [NBP3-31403] - Standard Curve Reference")
![ELISA: Human DGAT2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31404] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31404_human-dgat2-ready-to-use-elisa-kit-colorimetric-206202415311362.png "ELISA: Human DGAT2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31404] - Standard Curve Reference")
