DFF45/ICAD Antibody Pair

Product Discontinued

DFF45/ICAD Antibody Pair Summary

• Description: Quality control test: Standard curve using recombinant protein (H00001676-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human DFFA.
  
  
• Capture antibody: rabbit MaxPab affinity purified polyclonal anti-DFFA (100 ug)
• Detection antibody: mouse purified polyclonal anti-DFFA (20 ug)
• Standard Curve Range: 0.03 ng/ml to 100 ng/ml
• Assay Type: Sandwich ELISA
• Clonality: Monoclonal
• Gene: DFFA

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 0.03 ng/ml - 100 ng/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1x PBS, pH 7.4
• Preservative: No Preservative

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DFF45/ICAD Antibody Pair

• DFF1
• DFF1DNA fragmentation factor 45 kDa subunit
• DFF45
• DFF45DNA fragmentation factor subunit alpha
• DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
• DFFA
• DNA fragmentation factor, 45kDa, alpha polypeptide
• ICAD
• Inhibitor of CAD

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: DFFA