DFF45/ICAD Antibody (3A11) - Azide and BSA Free

Western Blot: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Analysis of DFFA expression in transfected 293T cell line by DFFA monoclonal antibody (M05), clone 3A11. Lane 1: DFFA transfected lysate (Predicted MW: 36.5 KDa). Lane 2: Non-transfected lysate.

Proximity Ligation Assay: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Analysis of protein-protein interactions between CASP3 and DFFA. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-DFFA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c

Sandwich ELISA: DFF45/ICAD Antibody (3A11) [H00001676-M05] - Detection limit for recombinant GST tagged DFFA is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func
• Clone: 3A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DFF45/ICAD Antibody (3A11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DFFA (NP_004392.1, 231 a.a. ~ 331 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
• Specificity: DFFA - DNA fragmentation factor, 45kDa, alpha polypeptide (3A11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DFFA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DFF45/ICAD Antibody (3A11) - Azide and BSA Free

• DFF1
• DFF1DNA fragmentation factor 45 kDa subunit
• DFF45
• DFF45DNA fragmentation factor subunit alpha
• DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
• DFFA
• DNA fragmentation factor, 45kDa, alpha polypeptide
• ICAD
• Inhibitor of CAD

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DFFA
• Entrez:
  • 1676(Human)
• Uniprot:
  • NP_004392(Human)