DFF40/CAD Antibody

Images

 
Western Blot: DFF40/CAD Antibody [NBP1-77009] - Analysis of 50 ug of murine lung tissue lysate with anti-CAD (I17) at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody [NBP1-77009] - Staining of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml.
Immunohistochemistry: DFF40/CAD Antibody [NBP1-77009] - Staining of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody [NBP1-77009] - Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 ug/mL.
Immunohistochemistry-Paraffin: DFF40/CAD Antibody [NBP1-77009] - Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 ug/mL.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DFF40/CAD Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD. The immunogen is located within amino acids 190 - 240 of CAD.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
Dffb
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
CAD antibody can be used for detection of CAD by Western blot at 0.5 - 1 mg/ml. A 40 kDa band should be detected.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DFF40/CAD Protein (NBP1-77009PEP)
Publications
Read Publications using NBP1-77009.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DFF40/CAD Antibody

  • CAD
  • CADCaspase-activated nuclease
  • Caspase-activated deoxyribonuclease
  • Caspase-activated DNase
  • CPAN
  • CPANDNA fragmentation factor 40 kDa subunit
  • DFF2
  • DFF40
  • DFF-40DFF40DFF2DNA fragmentation factor subunit beta
  • DFFB
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DFF40/CAD Antibody (NBP1-77009)(3)

Reviews for DFF40/CAD Antibody (NBP1-77009) (0)

There are no reviews for DFF40/CAD Antibody (NBP1-77009). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DFF40/CAD Antibody (NBP1-77009). (Showing 1 - 1 of 1 FAQs).

  1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
    • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol Dffb
Entrez
Uniprot