DFF40/CAD Antibody


Western Blot: DFF40/CAD Antibody [NBP1-76948] - Analysis of DFF40/CAD in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF40/CAD (I18) using NBP1-76948 at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody [NBP1-76948] - Jurkat cells with DFF40 antibody at 5 ug/ml.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

Order Details

DFF40/CAD Antibody Summary

Antibody was raised against a peptide corresponding to amino acids near the center of murine CAD. The sequence differs from human DFF40 by two amino acids (3,4).
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:1000 dilution. A 40 kDa band can be detected.
Control Peptide
DFF40/CAD Peptide (NBP1-76948PEP)

Reactivity Notes

Human, Mouse, Rat

Alternate Names for DFF40/CAD Antibody

  • CAD
  • CADCaspase-activated nuclease
  • Caspase-activated deoxyribonuclease
  • Caspase-activated DNase
  • CPAN
  • CPANDNA fragmentation factor 40 kDa subunit
  • DFF2
  • DFF-40DFF40DFF2DNA fragmentation factor subunit beta
  • DFFB
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • EC 3.-


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DFF40/CAD Antibody (NBP1-76948). (Showing 1 - 1 of 1 FAQ).

  1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
    • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol DFFB

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