| Reactivity | RtSpecies Glossary |
| Applications | ELISA |
| Clone | 023 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | HRP |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Rat Desmocollin-2 (Q3T1K6; Met1-Pro694). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DSC2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![E-Cadherin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "E-Cadherin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-90905AF350 | |
| Alexa Fluor 405 | NBP2-90905AF405 | |
| Alexa Fluor 488 | NBP2-90905AF488 | |
| Alexa Fluor 532 | NBP2-90905AF532 | |
| Alexa Fluor 594 | NBP2-90905AF594 | |
| Alexa Fluor 647 | NBP2-90905AF647 | |
| Alexa Fluor 700 | NBP2-90905AF700 | |
| Alexa Fluor 750 | NBP2-90905AF750 | |
| Biotin | NBP2-90905B | |
| DyLight 350 | NBP2-90905UV | |
| DyLight 405 | NBP2-90905V | |
| DyLight 488 | NBP2-90905G | |
| DyLight 550 | NBP2-90905R | |
| DyLight 594 | NBP2-90905DL594 | |
| DyLight 650 | NBP2-90905C | |
| DyLight 680 | NBP2-90905FR | |
| DyLight 755 | NBP2-90905IR | |
| FITC | NBP2-90905F | |
| HRP | NBP2-90905H |
Diseases for Desmocollin-2 Antibody (NBP2-90905H)Discover more about diseases related to Desmocollin-2 Antibody (NBP2-90905H).
| Pathways for Desmocollin-2 Antibody (NBP2-90905H)View related products by pathway.
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PTMs for Desmocollin-2 Antibody (NBP2-90905H)Learn more about PTMs related to Desmocollin-2 Antibody (NBP2-90905H).
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DSC2 |
![Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/Desmoplakin-Antibody-Immunohistochemistry-Paraffin-NBP1-49879-img0001.jpg)
![Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human skin shows moderate to strong membranous positivity in epidermal cells.](http://images.novusbio.com/images2/Desmocollin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88099-img0012.jpg)
![Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human fallopian tube shows very weak membranous positivity in glandular cells.](http://images.novusbio.com/images2/Desmocollin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88099-img0010.jpg)
![Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human prostate shows no membranous positivity in glandular cells.](http://images.novusbio.com/images2/Desmocollin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88099-img0011.jpg)
![Western Blot: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human cell lines Caco-2 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/Plakophilin-2-Antibody-Western-Blot-NBP1-86078-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Plakophilin 2 Antibody [NBP1-86078] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/images2/Plakophilin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86078-img0008.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-86078] - Analysis in human heart muscle and liver tissues using NBP1-86078 antibody. Corresponding Plakophilin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Plakophilin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86078-img0025.jpg)
![Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain](http://images.novusbio.com/images2/RCAN1 Antibody-Western Blot-NBP1-46853-img0003.jpg)
![Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain](http://images.novusbio.com/images2/RCAN1 Antibody-Immunohistochemistry-NBP1-46853-img0004.jpg)
![Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Desmoglein-3 Antibody (5H10)-Western Blot-NBP1-78984-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.](http://images.novusbio.com/images2/Desmoglein-3-Antibody-(5H10)-Immunocytochemistry-NBP1-78984-img0007.jpg)
![Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.](http://images.novusbio.com/images2/Desmoglein-3-Antibody-(5H10)-Immunohistochemistry-NBP1-78984-img0005.jpg)
![Western Blot: DSPP Antibody [NBP2-92546] - Analysis of extracts of various cell lines, using DSPP at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/images2/DSPP Antibody-Western Blot-NBP2-92546-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of U-2 OS cells using DSPP. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/DSPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92546-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DSPP Antibody [NBP2-92546] - Analysis of H9C2 cells using DSPP. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/DSPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92546-img0001.jpg)
![Western Blot: gamma Catenin Antibody (4C12) [NBP2-37557] - Western blot analysis using JUP mouse mAb against T47D (1), MCF-7 (2), SKBR-3 (3), A431 (4) and HEK293 (5) cell lysate.](http://images.novusbio.com/images2/gamma-Catenin-Antibody-(4C12)-Western-Blot-NBP2-37557-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: gamma Catenin Antibody (4C12) [NBP2-37557] - Immunofluorescence analysis of U251 cells using JUP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/gamma-Catenin-Antibody-(4C12)-Immunocytochemistry-Immunofluorescence-NBP2-37557-img0004.jpg)
![Immunohistochemistry: gamma Catenin Antibody (4C12) [NBP2-37557] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using JUP mouse mAb with DAB staining.](http://images.novusbio.com/images2/gamma-Catenin-Antibody-(4C12)-Immunohistochemistry-NBP2-37557-img0006.jpg)
![Immunohistochemistry-Paraffin: TMEM43 Antibody [NBP1-84132] - Staining of human skin shows moderate to strong positivity in nuclear membrane in keratinocytes.](http://images.novusbio.com/images2/TMEM43-Antibody-Immunohistochemistry-Paraffin-NBP1-84132-img0011.jpg)
![Immunohistochemistry-Paraffin: TMEM43 Antibody [NBP1-84132] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/images2/TMEM43-Antibody-Immunohistochemistry-Paraffin-NBP1-84132-img0009.jpg)
![Immunohistochemistry-Paraffin: TMEM43 Antibody [NBP1-84132] - Staining of human prostate shows weak positivity in nuclear membrane in glandular cells, as well as in smooth muscle cells.](http://images.novusbio.com/images2/TMEM43-Antibody-Immunohistochemistry-Paraffin-NBP1-84132-img0010.jpg)
![Western Blot: PTGDR Antibody [H00005729-B01P] - Analysis of PTGDR expression in transfected 293T cell line by PTGDR polyclonal antibody. Lane 1: PTGDR transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/PTGDR Antibody-Western Blot-H00005729-B01P-img0004.jpg)
![Flow Cytometry: PTGDR Antibody [H00005729-B01P] - Analysis of negative control 293 cells (Black) and PTGDR expressing 293 cells (Green) using PTGDR purified mouse polyclonal antibody.](http://images.novusbio.com/images2/PTGDR-Antibody-Flow-Cytometry-H00005729-B01P-img0003.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Immunohistochemistry-Paraffin: CDX2 Antibody [NB100-2136] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDX2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/CDX2-Antibody-Immunohistochemistry-Paraffin-NB100-2136-img0006.jpg)
![Immunoprecipitation: CDX2 Antibody [NB100-2136] - Detection of Human CDX2 on HeLa whole cell lysate using NB100-2136. CDX2 was also immunoprecipitated by rabbit anti-CDX2 antibody NB100-2137 using 3 mcg/mg lysate.](http://images.novusbio.com/images2/CDX2-Antibody-Immunoprecipitation-NB100-2136-img0003.jpg)
![Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis using Anti-PKP1 antibody NBP1-90042 (A) shows similar pattern to independent antibody NBP1-90041 (B).](http://images.novusbio.com/images2/Plakophilin 1 Antibody-Western Blot-NBP1-90042-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Plakophilin 1 Antibody [NBP1-90042] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/images2/Plakophilin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90042-img0005.jpg)
![Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining in human skin and skeletal muscle tissues using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Plakophilin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90042-img0008.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")