Desmocollin-1 Antibody (772906) [Unconjugated]

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Desmocollin-1 at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

B16‑F1 mouse melanoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

A549 human lung carcinoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Desmocollin-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Desmocollin-1 was detected in perfusion fixed frozen sections of mouse skin using Rat Anti-Human/Mouse Desmocollin-1 Monoclonal Antibody (Catalog # MAB7367) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to hair root sheath. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clone: 772906
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Desmocollin-1 Antibody (772906) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-1
  Arg135-Lys691
  Accession # P55849
• Specificity: Detects mouse Desmocollin-1 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Desmocollin-1 is observed, and no cross-reactivity with recombinant mouse Desmocollin-2 or -3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: DSC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-1 Antibody (772906) [Unconjugated]

• cadherin family member 1
• CDHF1
• desmocollin 1
• Desmocollin1
• Desmocollin-1
• desmosomal glycoprotein 2/3
• DG2/DG3
• DG2/DG3
• DSC1

Background

DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca⁺⁺-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin.
DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmocollin-1 Antibody (MAB7367)(2)

Publications using MAB7367

• BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality Sci Rep, 2016-08-26;6(0):32399. 2016-08-26 [PMID: 27561386] (IHC-Fr, Human)
• Kountikov E, Poe J, Maclver N, Rathmell J, Tedder T A spontaneous deletion within the desmoglein 3 extracellular domain of mice results in hypomorphic protein expression, immunodeficiency, and a wasting disease phenotype. Am J Pathol, 2014-12-24;185(3):617-30. 2014-12-24 [PMID: 25542773] (Western Blot, Mouse)

Review for Desmocollin-1 Antibody (MAB7367) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 04/18/2025

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Vagina
• Species: Mouse
• Lot: CHJD0123091

Comments

• Comments: Requires proteinase induced epitope retrieval with 5 min proteinase K at room temperature

Bioinformatics

• Gene Symbol: DSC1
• Uniprot:
  • P55849()