Desmocollin-1 Antibody (772906) [Unconjugated]

Images

 
Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line and B16‑F1 mouse melanoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody ...read more
A549 human lung carcinoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), ...read more
Desmocollin‑1 was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/MouseDesmocollin‑1 Monoclonal Antibody (Catalog # MAB7367) at 10 µg/mL for 3 hours at room temperature. ...read more
Desmocollin‑1 was detected in perfusion fixed frozen sections of mouse skin using Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
B16‑F1 mouse melanoma cell line was stained with Rat Anti-Human/Mouse Desmocollin‑1 Monoclonal Antibody (Catalog # MAB7367, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clone
772906
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Desmocollin-1 Antibody (772906) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-1
Arg135-Lys691
Accession # P55849
Specificity
Detects mouse Desmocollin-1 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Desmocollin-1 is observed, and no cross-reactivity with recombinant mouse Desmocollin-2 or -3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
DSC1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB7367 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-1 Antibody (772906) [Unconjugated]

  • cadherin family member 1
  • CDHF1
  • desmocollin 1
  • Desmocollin1
  • Desmocollin-1
  • desmosomal glycoprotein 2/3
  • DG2/DG3
  • DG2/DG3
  • DSC1

Background

DSC-1 (Desmocollin [Greek for "glue-that-binds"]-1) is an approximately 95-110 kDa member of the Ca++-dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including the spinous and granular layers of keratinized and nonkeratinized epithelia of the oral cavity and skin.
DSC‑1 expression is induced by DSG-1. It serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, homodimeric DSC-1 may form heterotypic interactions with DSG-1 in-trans, and bind to the cytoskeleton intracellularly via plakophilin-1. Mature mouse DSC-1 is a 760 amino acid (aa) type I transmembrane glycoprotein (aa 135-691). The mature molecule contains a 557 aa extracellular region with five cadherin domains (aa 135-682), and a 172 aa cytoplasmic domain. There is one splice variant that shows an 11 aa substitution for aa 822-886. Over aa 135-691, mouse DSC‑1 shares 82% aa sequence identity with human DSC-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmocollin-1 Antibody (MAB7367)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC Fresh, WB.


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(1)
WB
(1)
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Bioinformatics

Gene Symbol DSC1
Entrez
Uniprot