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Desmin Overexpression Lysate

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Western Blot: Desmin Overexpression Lysate (Adult Normal) [NBL1-09840] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Desmin.
Western Blot: Desmin Overexpression Lysate (Adult Normal) [NBL1-09840] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Desmin.
Western Blot: Desmin Overexpression Lysate (Adult Normal) [NBL1-09840] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DES

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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Desmin Overexpression Lysate Summary

Description

Desmin Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC205685

Accession#: NM_001927

Protein Tag: C-MYC/DDK
Gene
DES

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Desmin Overexpression Lysate

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
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Bioinformatics

Gene Symbol DES