Recombinant Human Defensin beta 2 Protein

SDS-Page: Human Defensin beta 2 Protein [NBP2-34910]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Defensin beta 2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 41 amino acids corresponding to Defensin beta 2 Source: E. coli

  Uniprot ID: O15263

  Amino Acid Sequence: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

• Details of Functionality: Defensin beta 2 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: Defb2
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Defensin beta 2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 4.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Defensin beta 2 Protein

• BD-2
• Defb2 defensin beta 2
• Defb2
• defensin beta 2

Background

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. B-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human B-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because B-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: Defb2
• Uniprot:
  • O15263()
  • O15263()
  • O15263()