Dectin-2/CLEC6A Antibody

Human blood-derived monocytes were stained with Goat Anti-Human Dectin‑2/CLEC6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3114, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Dectin-2/CLEC6A Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Dectin‑2/CLEC6A
  Thr46-Leu209
  Accession # Q6EIG7
• Specificity: Detects human Dectin‑2/CLEC6A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Dectin-2 alpha is observed and less than 1% cross-reactivity with recombinant human Dectin-1/CLEC7A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLEC6A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-2/CLEC6A Antibody

• CLEC4N
• CLEC6A
• CLECSF10
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
• C-type lectin domain family 6 member A
• C-type lectin domain family 6, member A
• C-type lectin superfamily member 10
• DC-associated C-type lectin 2
• dectin 2
• Dectin2
• Dectin-2
• Dendritic cell-associated C-type lectin 2

Background

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced beta isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kDa molecule that is expressed on monocytes, tissue macrophages, and activated CD4⁺ T cells (4‑6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 is up‑regulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4⁺CD25⁺ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (10).

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
3. Flornes, L.M. et al. (2004) Immunogenetics 56:506.
4. Kanazawa, N. et al. (2004) J. Invest. Dermatol. 122:1522.
5. Gavino, A.C. et al. (2005) Exp. Dermatol. 14:281.
6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
9. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
10. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dectin-2/CLEC6A Antibody (AF3114)(2)

Publications using AF3114

• Gaifem, J;Rodrigues, CS;Petralia, F;Alves, I;Leite-Gomes, E;Cavadas, B;Dias, AM;Moreira-Barbosa, C;Revés, J;Laird, RM;Novokmet, M;Štambuk, J;Habazin, S;Turhan, B;Gümü?, ZH;Ungaro, R;Torres, J;Lauc, G;Colombel, JF;Porter, CK;Pinho, SS; A unique serum IgG glycosylation signature predicts development of Crohn's disease and is associated with pathogenic antibodies to mannose glycan Nature immunology 2024-07-30 [PMID: 39080486] (Flow Cytometry, Human)
• JAT de Albuque, PP Banerjee, A Castoldi, R Ma, NB Zurro, LH Ynoue, C Arslanian, MUW Barbosa-Ca, JEM Correia-De, FG Weiler, MR Dias-da-Si, M Lazaretti-, LA Pedroza, NOS Câmara, E Mace, JS Orange, A Condino-Ne The Role of AIRE in the Immunity Against Candida Albicans in a Model of Human Macrophages Front Immunol, 2018-03-21;9(0):567. 2018-03-21 [PMID: 29666621] (Confocal Microscopy, Western Blot, Human)

Bioinformatics

• Gene Symbol: CLEC6A
• Uniprot:
  • Q6EIG7()