Decorin Antibody (115402) [Unconjugated]

Images

 
Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
115402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Decorin Antibody (115402) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Decorin
Gly17-Lys172
Accession # NP_598013.1
Specificity
Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Decorin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DCN
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB143 in the following applications:

Publications
Read Publications using
MAB143 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Decorin Antibody (115402) [Unconjugated]

  • Bone proteoglycan II
  • CSCD
  • DCN
  • decorin proteoglycan
  • Decorin
  • dermatan sulphate proteoglycans II
  • DSPG2
  • PG40
  • PGII
  • PG-II
  • PGS2
  • PG-S2
  • proteoglycan core protein
  • SLRR1B
  • SLRR1BPGS2
  • small leucine-rich protein 1B

Background

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

  1. Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
  2. Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  3. Danielson, K. et al. (1993) Genomics 15:146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Decorin Antibody (MAB143)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(5)
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Human
(9)
Mouse
(1)
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Showing Publications 1 - 10 of 10.
Publications using MAB143 Applications Species
Y Alania, J Creighton, LT Trevelin, CA Zamperini, AK Bedran-Rus Regional contribution of proteoglycans to the fracture toughness of the dentin extracellular matrix J Biomech, 2020;0(0):109633. 2020 [PMID: 32035660] (IHC, Human) IHC Human
K Katsumata, J Ishihara, K Fukunaga, A Ishihara, E Yuba, E Budina, JA Hubbell Conferring extracellular matrix affinity enhances local therapeutic efficacy of anti-TNF-alpha antibody in a murine model of rheumatoid arthritis Arthritis Res. Ther., 2019;21(1):298. 2019 [PMID: 31870429] (IHC-Fr, Human) IHC-Fr Human
N Chiarelli, G Carini, N Zoppi, M Ritelli, M Colombi Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome PLoS ONE, 2018;13(1):e0191220. 2018 [PMID: 29346445] (ICC, Human) ICC Human
G Wisowski, EM Ko?ma, T Bielecki, A Pude?ko, K Olczyk Dermatan sulfate is a player in the transglutaminase 2 interaction network PLoS ONE, 2017;12(2):e0172263. 2017 [PMID: 28199387] (Immunoprecipitation, Human) Immunoprecipitation Human
J Martínez-A, R Clifton-Bl, MP Molloy Proteomics of thyroid tumours provides new insights into their molecular composition and changes associated with malignancy Sci Rep, 2016;6(0):23660. 2016 [PMID: 27025787] (Western Blot, Human) Western Blot Human
Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu Possible dual role of decorin in abdominal aortic aneurysm. PLoS ONE, 2015;10(3):e0120689. 2015 [PMID: 25781946] (Western Blot, Human) Western Blot Human
Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta. J Bone Miner Res, 2015;30(8):1445-56. 2015 [PMID: 25656619] (Western Blot, Human) Western Blot Human
Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, Nurnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, Nurnberg P, Sugahara K, Mundlos S, Hoffmann K Faulty initiation of proteoglycan synthesis causes cardiac and joint defects. Am. J. Hum. Genet., 2011;89(1):15-27. 2011 [PMID: 21763480] (Western Blot, Human) Western Blot Human
Yates CC, Krishna P, Whaley D, Bodnar R, Turner T, Wells A Lack of CXC chemokine receptor 3 signaling leads to hypertrophic and hypercellular scarring. Am. J. Pathol., 2010;176(4):1743-55. 2010 [PMID: 20203286] (IHC-P, Mouse) IHC-P Mouse
Li X, Pennisi A, Yaccoby S Role of decorin in the antimyeloma effects of osteoblasts. Blood, 2008;112(1):159-68. 2008 [PMID: 18436739] (Western Blot, Human) Western Blot Human

Review for Decorin Antibody (MAB143) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB143:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Decorin MAB143
Enlarge
4
reviewed by:
Anonymous
IHC-P Human 04/11/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedskin
SpeciesHuman
LotECI0215041

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Recent Reviews

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Anonymous
04/11/2019
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol DCN
Uniprot