DEC2/SHARP1 Antibody

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DEC2/SHARP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human DEC2
  Ala101-Gly287
  Accession # Q9C0J9
• Specificity: Detects human DEC2 direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DEC1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BHLHE41
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DEC2/SHARP1 Antibody

• basic helix-loop-helix domain containing, class B, 3
• basic helix-loop-helix family, member e41
• BHLHB3
• BHLHB3class E basic helix-loop-helix protein 41
• BHLHE41
• Class B basic helix-loop-helix protein 3
• DEC2
• DEC2Enhancer-of-split and hairy-related protein 1
• Differentially expressed in chondrocytes protein 2
• SHARP-1
• SHARP1hDEC2

Background

DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BHLHE41
• Uniprot:
  • Q9C0J9()