DDX46 Antibody

Immunohistochemistry-Paraffin: DDX46 Antibody [NB100-57489] - Human breast carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1,000 (0.2ug/ml).

Immunoprecipitation: DDX46 Antibody [NB100-57489] - Detection of Human DDX46 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX46 antibody NB100-57489 used for IP at 3 mcg/mg lysate. DDX46 was also immunoprecipitated by rabbit anti-DDX46 antibody NB100-57490, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Mu
• Applications: IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DDX46 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 using the numbering given in entry NP_055644.2 (GeneID 9879).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX46
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX46 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
• DEAD box protein 46
• EC 3.6.1
• EC 3.6.4.13
• FLJ25329
• KIAA0801probable ATP-dependent RNA helicase DDX46
• MGC9936
• PRP5 homolog
• Prp5
• Prp5-like DEAD-box protein
• PRPF5
• RNA helicase

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX46
• Entrez:
  • 9879(Human)
• Uniprot:
  • NP_055644.2(Human)