RBM5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RBM5 Antibody [NBP1-83304] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: RBM5 Antibody [NBP1-83304] - Staining of human small intestine shows strong nuclear and cytoplasmic positivity in glandular cells.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RBM5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBM5 Antibody - BSA Free (NBP1-83304) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RBM5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSDRSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSRGFAFVEFYHL
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RBM5 Protein (NBP1-83304PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25586139).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RBM5 Antibody - BSA Free

• G15
• H37
• LUCA15FLJ39876
• Protein G15
• Putative tumor suppressor LUCA15
• Renal carcinoma antigen NY-REN-9
• RMB5
• RNA binding motif protein 5
• RNA-binding motif protein 5
• RNA-binding protein 5

Background

RNA-binding motif protein 5 (RBM5) is also known as LUCA-15. The RBM5 gene is located within a region highly susceptible to deletions in lung cancer and is implicated to be a tumor suppressor. RBM5 bears two RRM domains, one RanBP2-type zinc finger, one C2H2-type zinc finger, and a G-patch domain. It has been found to be a component of the spliceosome, a complex that regulates alternative splicing and is involved in the regulation of proliferation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBM5 Antibody (NBP1-83304)(1)

Publication using NBP1-83304

• Travis C Jackson, Lina Du, Keri Janesko-Feldman et al. The nuclear splicing factor RNA binding motif 5 promotes caspase activation in human neuronal cells, and increases after traumatic brain injury in mice. Journal of Cerebral Blood Flow & Metabolism 2015-01-14 [PMID: 25586139] (WB, IF/IHC, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: RBM5