DDX3Y Antibody

Images

 
Western Blot: DDX3Y Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000
Immunocytochemistry/ Immunofluorescence: DDX3Y Antibody [NBP1-32780] - Analysis of paraformaldehyde-fixed HeLa, using DDX3Y antibody at 1:200 dilution.
Western Blot: DDX3Y Antibody [NBP1-32780] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: DDX3Y Antibody [NBP1-32780] - 50 ug Rat testis lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: DDX3Y Antibody [NBP1-32780] - Sample (30 ug of whole cell lysate)A: H1299B: Hep G2 7. 5% SDS PAGE, antibody diluted at 1:10000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DDX3Y Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Rhesus Macaque (99%), Bovine (94%), Primate (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX3Y
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for DDX3Y Antibody

  • ATP-dependent RNA helicase DDX3Y
  • DBYEC 3.6.4.13
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked
  • DEAD box protein 3, Y-chromosomal
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome
  • EC 3.6.1

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDX3Y