DDX3 Antibody

Images

 
Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-16133] - Analysis of paraformaldehyde-fixed Human ESC, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-16133] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.
Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of cell lysate) A: HeLaB: HeLa cytoplasm7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-16133] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.97 mg/ml

Order Details

DDX3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary.
Localization
Nucleus speckle; Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX3X
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (97%), Xenopus tropicalis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.97 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX3 Antibody

  • DBXCAP-Rf
  • DDX14
  • DDX3DEAD/H box-3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
  • DEAD box protein 3, X-chromosomal
  • DEAD box, X isoform
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
  • EC 3.6.1
  • EC 3.6.4.13
  • helicase like protein 2
  • Helicase-like protein 2
  • HLP2ATP-dependent RNA helicase DDX3X

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX3X
Uniprot