DDX27 Antibody - BSA Free

Immunohistochemistry-Paraffin: DDX27 Antibody [NBP1-26601] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DDX27 used at a dilution of 1:5,000 ( 0.2ug/ml) and 1:1,000 ( 1ug/ml). Detection: DAB

Immunoprecipitation: DDX27 Antibody [NBP1-26601] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit used for IP at 10 mcg/mg lysate.

• Reactivity: Hu, Mu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDX27 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX27 Antibody - BSA Free (NBP1-26601) is a polyclonal antibody validated for use in IHC and IP. Anti-DDX27 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 5-10 ug/mg lysate
• Application Notes: It is not recommended for western analysis. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX27 Antibody - BSA Free

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
• DEAD box protein 27
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27
• dJ686N3.1
• DKFZp667N057
• DRS1
• EC 3.6.1
• EC 3.6.4.13
• FLJ12917
• FLJ20596
• FLJ22238
• MGC1018
• MGC163147
• PP3241
• probable ATP-dependent RNA helicase DDX27
• RHLP
• RNA helicase-like protein
• Rrp3p

Background

DDX27 (DEAD-box protein 27) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX27 has not been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX27 Antibody (NBP1-26601)(1)

Publication using NBP1-26601

• Li S, Ma J, Zheng A Et al. DEAD-box helicase 27 enhances stem cell-like properties with poor prognosis in breast cancer Journal of translational medicine 2021-08-06 [PMID: 34362383] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: DDX27
• Entrez:
  • 55661(Human)
• Uniprot:
  • NP_060365.6(Human)