DDX1 Antibody

Western Blot: DDX1 Antibody [H00001653-A01] - Analysis of DDX1 expression in U-2 OS (Cat # L022V1).

Product Discontinued

DDX1 Antibody Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DDX1 (NP_004930, 642 a.a. - 740 a.a.) partial recombinant protein with GST tag. RLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF
• Specificity: DDX1 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DDX1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29731414).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50 % glycerol
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX1 Antibody

• ATP-dependent RNA helicase DDX1
• DBP-RB
• DBP-RBDEAD box polypeptide 1
• DDX1
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
• DEAD box protein 1
• DEAD box protein retinoblastoma
• DEAD Box-1
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
• DEAD/H-Box Helicase 1
• EC 3.6.1
• EC 3.6.1.10
• EC 3.6.4.13
• UKVH5d

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX1 Antibody (H00001653-A01)(1)

Publication using H00001653-A01

• Ribeiro de Almeida Claudia, Dhir Somdutta, Dhir Ashish et al. RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination. Oncology Reports 2018-05-17 [PMID: 29731414] (WB, Mouse)

Bioinformatics

• Gene Symbol: DDX1
• Entrez:
  • 1653(Human)
• OMIM:
  • 601257(Human)
• Uniprot:
  • NP_004930(Human)