DDOST Antibody (OTI2B4) - Azide and BSA Free

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Western Blot: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST.
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: DDOST Antibody (OTI2B4) - Azide and BSA Free [NBP2-70561] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

DDOST Antibody (OTI2B4) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDOST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
46.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for DDOST Antibody (OTI2B4) - Azide and BSA Free

  • advanced glycation endproduct receptor 1
  • DDOST 48 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase
  • dolichyl-diphosphooligosaccharide-protein glycotransferase
  • EC 2.4.1.119
  • KIAA0115MGC2191
  • Oligosaccharyl transferase 48 kDa subunit
  • oligosaccharyltransferase 48 kDa subunit
  • OST48AGER1
  • OSTOKSWcl45
  • WBP1

Background

DDOST encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDOST