Dcp1a Antibody (JB51-34)

Western Blot: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (JB51-34) [NBP2-75463] - Staining Dcp1a in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Dcp1a antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of LOVO cells with Dcp1a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (JB51-34) [NBP2-75463] - Staining Dcp1a in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (JB51-34) [NBP2-75463] - Staining Dcp1a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of paraffin-embedded human placenta tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of paraffin-embedded human liver tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Dcp1a Antibody (JB51-34) [NBP2-75463] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Dcp1a antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Dcp1a Antibody (JB51-34) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Dcp1a aa 460-582 / 582. (SwissProt: Q9NPI6 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DCP1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dcp1a Antibody (JB51-34)

• DCP1 decapping enzyme homolog A (S. cerevisiae)
• decapping enzyme hDcp1a
• EC 3.-
• FLJ21691
• mRNA-decapping enzyme 1A
• Nbla00360
• putative protein product of Nbla00360
• Smad4-interacting transcriptional co-activator
• SMAD4IP1
• SMIFHSA275986
• Transcription factor SMIF

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCP1A