DARC Antibody - BSA Free

Western Blot: DARC Antibody - BSA Free [NBP1-77278] - DARC Antibody [NBP1-77278] - DARC expression in monocytes is dependent on an IL-22/IL-22Ra1-STAT5b signaling axis. THP1 cells were incubated with 20 ng/mL IL-22 in the absence or presence of the indicated concentrations of the STAT5b inhibitor, CAS285989, for 24 h and DARC protein expression was determined by immunoblotting assay. Western blot was quantified and expressed as the ratio of DARC and beta-actin intensity. Data are representative of three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2073-4409/8/12/1587) licensed under a CC-BY license.

Western Blot: DARC Antibody - BSA Free [NBP1-77278] - DARC Antibody [NBP1-77278] - Analysis in human cerebellum tissue lysate with antibody at 1 ug/mL.

Immunohistochemistry: DARC Antibody - BSA Free [NBP1-77278] - Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DARC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DARC Antibody - BSA Free (NBP1-77278) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DARC Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC. The immunogen is located within the last 50 amino acids of DARC. Amino Acid Squence: LPEGWSSHLDTLGSKS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACKR1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  DARC Peptide (NBP1-77278PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DARC Antibody - BSA Free

• ACKR1
• CCBP1
• CD234 antigen
• CD234
• DARC
• Dfy
• Duffy Antigen
• Duffy antigen/chemokine receptor
• Duffy blood group antigen
• Duffy blood group, chemokine receptor
• Fy Antigen
• Fy glycoprotein
• FYDuffy blood group
• Glycoprotein D
• GPD
• GPDWBCQ1
• GpFy
• Plasmodium vivax receptor

Background

DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARC Antibody (NBP1-77278)(3)

Publications using NBP1-77278

• Kim EY, Noh HM, Choi B et al. Interleukin-22 Induces the Infiltration of Visceral Fat Tissue by a Discrete Subset of Duffy Antigen Receptor for Chemokine-Positive M2-Like Macrophages in Response to a High Fat Diet Cells 2019-12-06 [PMID: 31817755] (WB, Human)
• Gardner L, Patterson AM, Ashton BA et al. The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun. 321:306-12. 2004-01-01 [PMID: 15358176]
• Chaudhuri A, Polyakova J, Zbrzezna V et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA; 90:10793-7. 1993-01-01 [PMID: 8248172]

Bioinformatics

• Gene Symbol: ACKR1
• Entrez:
  • 2532(Human)
• Uniprot:
  • Q16570()