| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB6695P | |||
| Carrier Free | AF6695-SP | |||
| Carrier Free | FAB6695A-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77278-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75197-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21980-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86586-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86587-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75196-0.025mg | |||
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Western Blot: DARC Antibody - BSA Free [NBP1-77278] - DARC Antibody [NBP1-77278] - DARC expression in monocytes is dependent on an IL-22/IL-22Ra1-STAT5b signaling axis. THP1 cells were incubated with 20 ng/mL IL-22 in the absence or presence of the indicated concentrations of the STAT5b inhibitor, CAS285989, for 24 h and DARC protein expression was determined by immunoblotting assay. Western blot was quantified and expressed as the ratio of DARC and beta-actin intensity. Data are representative of three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2073-4409/8/12/1587) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NBP1-77278-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: DARC Antibody - BSA Free [NBP1-77278] - DARC Antibody [NBP1-77278] - DARC expression in monocytes is dependent on an IL-22/IL-22Ra1-STAT5b signaling axis. THP1 cells were incubated with 20 ng/mL IL-22 in the absence or presence of the indicated concentrations of the STAT5b inhibitor, CAS285989, for 24 h and DARC protein expression was determined by immunoblotting assay. Western blot was quantified and expressed as the ratio of DARC and beta-actin intensity. Data are representative of three independent experiments with similar results. Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2073-4409/8/12/1587) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: DARC Antibody - BSA Free [NBP1-77278] - DARC Antibody [NBP1-77278] - Analysis in human cerebellum tissue lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NBP1-77278-img0004.jpg)
![Immunohistochemistry: DARC Antibody - BSA Free [NBP1-77278] - Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77278_rabbit-polyclonal-darc-antibody-2711202419553068.jpg)
![Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.](http://images.novusbio.com/fullsize/DARC-Antibody-2C3-Chimeric-Western-Blot-NBP2-75197-img0001.jpg "Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.")
![Immunocytochemistry /Immunofluorescence: DARC Antibody (2C3) - Chimeric - Azide and BSA Free [NBP2-75197] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with NBP2-75197 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-75197_rabbit-monoclonal-darc-antibody-2c3-chimeric-74202522113041.png)
![Western Blot: DARC Antibody (SR1582) [NBP3-21980] - Western blot analysis using NBP3-21980 in Human fetal liver lysate.](http://images.novusbio.com/fullsize/nbp3-21980_rabbit-darc-mab-sr1582-57202317215170.jpg "Western Blot: DARC Antibody (SR1582) [NBP3-21980] - Western blot analysis using NBP3-21980 in Human fetal liver lysate.")
![Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86586] - Staining in human smooth muscle and kidney tissues using anti-ACKR1 antibody. Corresponding ACKR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86586-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86586] - Staining in human smooth muscle and kidney tissues using anti-ACKR1 antibody. Corresponding ACKR1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86586] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86586-img0005.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86586] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86586-img0004.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86587] - Staining in human smooth muscle and kidney tissues using anti-ACKR1 antibody. Corresponding ACKR1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86587-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86587] - Staining in human smooth muscle and kidney tissues using anti-ACKR1 antibody. Corresponding ACKR1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86587] - Staining of human smooth muscle shows high expression.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86587-img0005.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NBP1-86587] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NBP1-86587-img0004.jpg)
![SDS-Page: Recombinant Human DARC Protein [H00002532-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00002532-P01-1.jpg "SDS-Page: Recombinant Human DARC Protein [H00002532-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: DARC Overexpression Lysate (Adult Normal) [NBP2-09412] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARC.](http://images.novusbio.com/fullsize/DARC-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09412-img0001.jpg "Western Blot: DARC Overexpression Lysate (Adult Normal) [NBP2-09412] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARC.")
![Western Blot: DARC Overexpression Lysate (Adult Normal) [NBP2-04213] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARC.](http://images.novusbio.com/fullsize/DARC-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04213-img0001.jpg "Western Blot: DARC Overexpression Lysate (Adult Normal) [NBP2-04213] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DARC.")