DAP3 Antibody (42C617.1.2)

Images

 
Western Blot: DAP3 Antibody (42C617.1.2) [NB100-56539] - Analysis of DAP-3 in A) human brain, B) Jurkat and C) NIH 3T3 lysate using this antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
42C617.1.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

DAP3 Antibody (42C617.1.2) Summary

Immunogen
The antibody was raised against a synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein.
Specificity
In Jurkat, a 45 kDa band is observed.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
DAP3
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 3-5 ug/ml
Positive Control
Jurkat Lysate (NB800-PC2)
DAP3 Lysate (NBL1-09715)

Reactivity Notes

Reacts with Mouse and Human. 75% sequence identity with rat protein.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for DAP3 Antibody (42C617.1.2)

  • bMRP-10
  • DAP-3Death-associated protein 3
  • death associated protein 3
  • DKFZp686G12159,28S ribosomal protein S29, mitochondrial
  • FLJ12817
  • MGC126058
  • MGC126059
  • mitochondrial 28S ribosomal protein S29
  • MRP-S29Ionizing radiation resistance conferring protein
  • MRPS29S29mt

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DAP3 Antibody (NB100-56539) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56539AF405
Alexa Fluor 488 Labeled NB100-56539AF488
Alexa Fluor 647 Labeled NB100-56539AF647
Alexa Fluor 700 Labeled NB100-56539AF700
Biotin Labeled NB100-56539B
DyLight 350 Labeled NB100-56539UV
DyLight 405 Labeled NB100-56539V
DyLight 488 Labeled NB100-56539G
DyLight 550 Labeled NB100-56539R
DyLight 650 Labeled NB100-56539C
DyLight 680 Labeled NB100-56539FR
DyLight 755 Labeled NB100-56539IR
HRP Labeled NB100-56539H

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Bioinformatics

Gene Symbol DAP3
Entrez
Uniprot