DAP10/HCST Antibody

Product Discontinued

DAP10/HCST Antibody Summary

• Immunogen: Peptide with sequence C-RSPAQEDGKVYIN corresponding to C-Terminus according to NP_001007470.1.
• Epitope: RSPAQEDGKVYIN
• Specificity: This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HCST
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human spleen lysates is reported in scientific literature (PMID: 10426994). An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  DAP10/HCST Overexpression Lysate
  Human Spleen Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAP10/HCST Antibody

• DAP10
• DAP10PIK3AP
• DKFZP586C1522
• HCST
• hematopoietic cell signal transducer
• KAP10
• KAP10DNAX-activation protein 10
• kinase assoc pro of ~10kDa
• Kinase Assoc Protein
• Membrane protein DAP10
• phosphoinositide-3-kinase adaptor protein
• PIK3AP
• Transmembrane adapter protein KAP10

Background

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP10/HCST Antibody (NB100-55403)(1)

Publication using NB100-55403

• Upshaw JL, Arneson LN, Schoon RA et al. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol 2006-05-01 [PMID: 16582911]

Bioinformatics

• Gene Symbol: HCST
• Entrez:
  • 10870(Human)
  • 23900(Mouse)
  • 474146(Rat)
• Uniprot:
  • NP_055081.1(Human)