DAP10/HCST Antibody

Images

 
Western Blot: HCST Antibody [NB100-55403] - (0.2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DAP10/HCST Antibody Summary

Immunogen
Peptide with sequence C-RSPAQEDGKVYIN corresponding to C-Terminus according to NP_001007470.1.
Epitope
RSPAQEDGKVYIN
Specificity
This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HCST
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 40 kDa band observed in human spleen lysates is reported in scientific literature (PMID: 10426994). An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
DAP10/HCST Lysate (NBL1-11475)
Spleen Lysate (NB820-59259)
Publications
Read Publication using NB100-55403.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAP10/HCST Antibody

  • DAP10
  • DAP10PIK3AP
  • DKFZP586C1522
  • HCST
  • hematopoietic cell signal transducer
  • KAP10
  • KAP10DNAX-activation protein 10
  • kinase assoc pro of ~10kDa
  • Kinase Assoc Protein
  • Membrane protein DAP10
  • phosphoinositide-3-kinase adaptor protein
  • PIK3AP
  • Transmembrane adapter protein KAP10

Background

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP10/HCST Antibody (NB100-55403)(1)

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol HCST
Entrez
Uniprot