![Western Blot: DAAM2 Antibody [NBP2-92809] - Analysis of extracts of various cell lines, using DAAM2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize2/DAAM2-Antibody-Western-Blot-NBP2-92809-img0002.jpg "Western Blot: DAAM2 Antibody [NBP2-92809] - Analysis of extracts of various cell lines, using DAAM2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-86 of human DAAM2 (NP_056160.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DAAM2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 123 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry-Paraffin: FMNL2 Antibody [NBP2-24748] - Analysisof human brain, cortex using this antibody at 10 ug/ml.](http://images.novusbio.com/images2/FMNL2-Antibody-Immunohistochemistry-Paraffin-NBP2-24748-img0007.jpg)
![Immunohistochemistry-Paraffin: FMNL2 Antibody [NBP2-24748] - Staining of normal human brain using this antibody (FMNL2, figure A and control rabbit Ig, figure B) at 6 ug/ml.](http://images.novusbio.com/images2/FMNL2-Antibody-Immunohistochemistry-Paraffin-NBP2-24748-img0008.jpg)
![Western Blot: FMNL2 Antibody [NBP2-24748] - Analysis of Formin-like protein 2 in whole mouse embryo body tissue lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 2 ug/ml.](http://images.novusbio.com/images2/FMNL2-Antibody-Western-Blot-NBP2-24748-img0009.jpg)
![Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-33431] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/FMNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-33431-img0002.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - H1299 lysates (Lane A) and Hela cells (Lane B) using NBP1-33431 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.](http://images.novusbio.com/images2/FMNL1-Antibody-Western-Blot-NBP1-33431-img0001.jpg)
![Western Blot: FMNL1 Antibody [NBP1-33431] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/images2/FMNL1-Antibody-Western-Blot-NBP1-33431-img0003.jpg)
![Immunohistochemistry-Paraffin: GRID2IP Antibody [NBP1-94174] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/images2/GRID2IP-Antibody-Immunohistochemistry-Paraffin-NBP1-94174-img0002.jpg)
![Western Blot: GRID2IP Antibody [NBP1-94174] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/images2/GRID2IP-Antibody-Western-Blot-NBP1-94174-img0004.jpg)
![ELISA: FMN1 Antibody (4F4) [H00342184-M07] - Detection limit for recombinant GST tagged FMN1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-ELISA-H00342184-M07-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of monoclonal antibody to FMN1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-Immunocytochemistry-Immunofluorescence-H00342184-M07-img0001.jpg)
![Western Blot: FMN1 Antibody (4F4) [H00342184-M07] - Analysis of FMN1 expression in human colon.](http://images.novusbio.com/images2/FMN1-Antibody-(4F4)-Western-Blot-H00342184-M07-img0004.jpg)
![Western Blot: FNBP3 Antibody [NBP1-87933] - Analysis using Anti-PRPF40A antibody NBP1-87933 (A) shows similar pattern to independent antibody NBP2-38400 (B).](http://images.novusbio.com/images2/FNBP3-Antibody-Western-Blot-NBP1-87933-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FNBP3 Antibody [NBP1-87933] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/images2/FNBP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87933-img0009.jpg)
![Immunohistochemistry-Paraffin: FNBP3 Antibody [NBP1-87933] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/images2/FNBP3-Antibody-Immunohistochemistry-Paraffin-NBP1-87933-img0002.jpg)
![Western Blot: FMN2 Antibody [NBP2-24615] - Analysis of FMN2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24615 at 0.25 ug/ml.](http://images.novusbio.com/images2/FMN2-Antibody-Western-Blot-NBP2-24615-img0001.jpg)
![Immunohistochemistry-Paraffin: FMN2 Antibody [NBP2-24615] - Tissue section of human brain using 1:300 dilution of FMN2 antibody. The signal was developed using HRP-labeled secondary and DAB reagent which followed counterstaining of nuclei with hematoxylin. This FMN2 antibody generated a nuclear and cytoplasmic staining primarily in the glial cells.](http://images.novusbio.com/images2/FMN2-Antibody-Immunohistochemistry-Paraffin-NBP2-24615-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/DIAPH3-Antibody-(4D5)-Immunocytochemistry-Immunofluorescence-H00081624-M01-img0002.jpg)
![Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/DIAPH3-Antibody-(4D5)-Western-Blot-H00081624-M01-img0004.jpg)
![Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/images2/DIAPH3-Antibody-(4D5)-Sandwich-ELISA-H00081624-M01-img0003.jpg)
![Immunohistochemistry-Paraffin: FNBP1 Antibody [NBP2-48542] - Staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.](http://images.novusbio.com/images2/FNBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-48542-img0002.jpg)
![Immunohistochemistry-Paraffin: FNBP1 Antibody [NBP2-48542] - Staining of human lymph node shows high expression.](http://images.novusbio.com/images2/FNBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-48542-img0003.jpg)
![Western Blot: FNBP1 Antibody [NBP2-48542] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/images2/FNBP1-Antibody-Western-Blot-NBP2-48542-img0004.jpg)
![Immunohistochemistry-Paraffin: WBP4 Antibody [NBP1-84718] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/images2/WBP4-Antibody-Immunohistochemistry-Paraffin-NBP1-84718-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: WBP4 Antibody [NBP1-84718] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/images2/WBP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84718-img0008.jpg)
![Western Blot: WBP4 Antibody [NBP1-84718] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/WBP4-Antibody-Western-Blot-NBP1-84718-img0007.jpg)
![Flow Cytometry: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/Cortactin-Antibody-(SC61-08)-Flow-Cytometry-NBP2-67632-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Cortactin-Antibody-(SC61-08)-Immunocytochemistry-Immunofluorescence-NBP2-67632-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Cortactin-Antibody-(SC61-08)-Immunocytochemistry-Immunofluorescence-NBP2-67632-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for DAAM2 Antibody (NBP2-92809)Discover more about diseases related to DAAM2 Antibody (NBP2-92809).
| Pathways for DAAM2 Antibody (NBP2-92809)View related products by pathway.
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PTMs for DAAM2 Antibody (NBP2-92809)Learn more about PTMs related to DAAM2 Antibody (NBP2-92809).
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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| Gene Symbol | DAAM2 |
![Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of H9C2 cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/DAAM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92809-img0005.jpg "Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of H9C2 cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of U2OS cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/DAAM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92809-img0003.jpg "Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of U2OS cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of L929 cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/DAAM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92809-img0004.jpg "Immunocytochemistry/Immunofluorescence: DAAM2 Antibody [NBP2-92809] - Analysis of L929 cells using DAAM2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/DAAM1-Antibody-(4H3)-ELISA-H00023002-M03-img0003.jpg)
![Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/images2/DAAM1-Antibody-(4H3)-Proximity-Ligation-Assay-H00023002-M03-img0004.jpg)
![Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/images2/DAAM1-Antibody-(4H3)-Western-Blot-H00023002-M03-img0005.jpg)
![Western Blot: FMNL3 Antibody [NBP2-24724] - Analysis of Formin-like protein 3 in human brain lysate using this antibody. 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/FMNL3-Antibody-Western-Blot-NBP2-24724-img0003.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-Diaphanous1 used at a dilution of 1:100.](http://images.novusbio.com/images2/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NB600-231-img0006.jpg)
![Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/images2/DIAPH1-Antibody-Western-Blot-NB600-231-img0008.jpg)
![Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/images2/DIAPH1-Antibody-Western-Blot-NB600-231-img0009.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/images2/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0013.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining of human placenta shows high expression.](http://images.novusbio.com/images2/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0014.jpg)
![Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP1-85217] - Staining in human placenta and pancreas tissues using anti-DIAPH2 antibody. Corresponding DIAPH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/DIAPH-2-Antibody-Immunohistochemistry-Paraffin-NBP1-85217-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: FHOD1 Antibody [NBP1-83900] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/FHOD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83900-img0004.jpg)
![Immunohistochemistry-Paraffin: FHOD1 Antibody [NBP1-83900] - Staining of human cerebellum shows no cytoplasmic positivity in Purkinje cells as expected.](http://images.novusbio.com/images2/FHOD1-Antibody-Immunohistochemistry-Paraffin-NBP1-83900-img0005.jpg)
![Immunohistochemistry-Paraffin: FHOD1 Antibody [NBP1-83900] - Staining of human gastrointestinal shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/images2/FHOD1-Antibody-Immunohistochemistry-Paraffin-NBP1-83900-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Filamin B Antibody [NBP1-90286] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Filamin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90286-img0015.jpg)
![Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in human cell lines A-549 and HEK293 using anti-FLNB antibody. Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/images2/Filamin-B-Antibody-Western-Blot-NBP1-90286-img0017.jpg)
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon, placenta, prostate and skeletal muscle using Anti-Filamin B antibody NBP1-90286 (A) shows similar protein distribution across tissues to independent antibody NBP1-90285 (B).](http://images.novusbio.com/images2/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0027.jpg)
![Immunohistochemistry-Paraffin: FHDC1 Antibody [NBP1-93579] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/FHDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-93579-img0001.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")