DAAM1 Antibody (4H3)

Images

 
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).
Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3. Lane 1: DAAM1 transfected lysatE (122.3 KDa). Lane 2: ...read more
ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.
Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, PLA
Clone
4H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

DAAM1 Antibody (4H3) Summary

Immunogen
DAAM1 (NP_055807, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag.MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQL
Specificity
DAAM1 (4H3)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DAAM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Proximity Ligation Assay
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DAAM1 Antibody (4H3)

  • disheveled-associated activator of morphogenesis 1
  • dishevelled associated activator of morphogenesis 1
  • dishevelled-associated activator of morphogenesis 1
  • KIAA0666FLJ41657

Background

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAAM1
Entrez
OMIM
Uniprot