DAAM1 Antibody (4H3) - Azide and BSA Free

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in A-431 (Cat # L015V1).

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in PC-12 (Cat # L012V1).

Western Blot: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody (M03), clone 4H3. Lane 1: DAAM1 transfected lysatE (122.3 KDa). Lane 2: Non-transfected lysate.

ELISA: DAAM1 Antibody (4H3) [H00023002-M03] - Detection limit for recombinant GST tagged DAAM1 is approximately 1ng/ml as a capture antibody.

Proximity Ligation Assay: DAAM1 Antibody (4H3) [H00023002-M03] - Analysis of protein-protein interactions between RHOA and DAAM1. HeLa cells were stained with anti-RHOA rabbit purified polyclonal 1:1200 and anti-DAAM1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Func, IP
• Clone: 4H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DAAM1 Antibody (4H3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DAAM1 Antibody (4H3) - Azide and BSA Free (H00023002-M03) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-DAAM1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DAAM1 (NP_055807, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQL
• Specificity: DAAM1 (4H3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAAM1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunoprecipitation reported in scientific literature (PMID: 29972794).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29972794).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DAAM1 Antibody (4H3) - Azide and BSA Free

• disheveled-associated activator of morphogenesis 1
• dishevelled associated activator of morphogenesis 1
• dishevelled-associated activator of morphogenesis 1
• KIAA0666FLJ41657

Background

Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAAM1 Antibody (H00023002-M03)(1)

Publication using H00023002-M03

• kawabata Galbraith k, Fujishima k, Mizuno H et al. MTSS1 Regulation of Actin-Nucleating Formin DAAM1 in Dendritic Filopodia Determines Final Dendritic Configuration of Purkinje Cells Cell Rep. 2018-07-03 [PMID: 29972794] (WB, IP, Mouse)

Bioinformatics

• Gene Symbol: DAAM1
• Entrez:
  • 23002(Human)
• OMIM:
  • 606626(Human)
• Uniprot:
  • NP_055807(Human)