Cytosolic beta-Glucosidase/GBA3 Overexpression Lysate (Native)

Images

 
Western Blot: GBA3 Overexpression Lysate (Adult Normal) [NBL1-10992] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GBA3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cytosolic beta-Glucosidase/GBA3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211035 and based on accession number NM_020973. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glucosidase, beta, acid 3 (cytosolic) (GBA3), transcript variant 1, mRNA.
Gene
GBA3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cytosolic beta-Glucosidase/GBA3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cytosolic beta-Glucosidase/GBA3 Overexpression Lysate (Native)

  • CBG
  • CBGL1
  • CBGL1GLUC
  • Cytosolic betaGlucosidase
  • Cytosolic beta-Glucosidase
  • Cytosolic beta-glucosidase-like protein 1
  • EC 3.2.1.21
  • GBA3
  • GLUC
  • glucosidase, beta, acid 3 (cytosolic)
  • klotho-related protein
  • KLrP
  • MGC104276
  • MGC126878

Background

GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GBA3
Entrez