Cytokeratin, pan Antibody

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Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Cytokeratin, pan Antibody Summary

Immunogen
This Cytokeratin, pan Antibody was developed against pan Cytokeratin isolated from bovine muzzle epidermis.
Localization
Cytoplasmic
Marker
Epithelial marker
Specificity
NB600-579 has cross-reactivity with cytokeratins of 58, 56, 52, 60, 51, 48 and 68 kDa molecular weight. The antibody is well suited for the staining of a broad spectrum of human keratins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Frozen 1:75 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
IHC-P: recommended pretreatment of HistoZyme. Recommended incubation time of 30 min at RT.
Control
Human Skin Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB600-579 in the following applications:

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 28916434). Mouse reactivity reported in scientific literature (PMID: 32421534).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Cytokeratin, pan Antibody

  • AEI2;CK1;EHK;EHK1;EPPK;K1;Keratin, type II cytoskeletal 1;KRT1A;NEPPK

Background

Cytokeratins are a family of intermediate filamentous proteins that are expressed by epithelial cells (1,2). Cytokeratins range in size with a theoretic molecular weight varying from approximately 40 kDa to 68 kDa (2,3). The cytokeratin family consists of 20 polypeptides that are further divided into two main groups based on isoelectric point and molecular weight (1-3). The type I group are smaller, acidic polypeptides designated as cytokeratin 9 through cytokeratin 20 (CK9 - CK20) (1-4). Conversely, CK1 - CK8 belong to the type II group, classified as larger, basic or neutral polypeptides (1-4). Structurally, cytokeratins have homologous basic structure with other intermediate filaments; they possess a 300-315 amino acid (aa) central helical region that consists of four conserved domains (1A, 2A, 1B, and 2B) which are separated by linker domains (L1, L12, and L2) (1,5). Additionally, flanking this central region, both the amino- and carboxyl-terminal ends have a homologous subdomain (H), a variable domain (V), and charged end subdomains (E) (1). Furthermore, the central rod of one cytokeratin monomer binds with another monomer to form a coiled-coil dimer which subsequently binds another dimer to form a tertramer (3). Finally, many tetramers join together to ultimately form an intermediate filament of approximately 10nm in diameter (1-3, 5). Cytokeratins are expressed as pairs, typically with a type I and type II member; for example, CK10 pairs with CK1 (1,3).

Epithelial cells express multiple subtypes of cytokeratins which can be used to classify epithelial cell type or differentiation status, as well tumor progression or diagnosis (2). Cytokeratins are important for both stability and integrity of epithelial cells and function in intracellular signaling, from wound healing to apoptosis (1). Cytokeratins are useful immunohistochemistry tumor markers and antibodies to cytokeratins are a common pathological tool (1,3,6). Cytokeratin pan antibody is an antibody cocktail mixture that can detect multiple cytokeratins and reacts to multiple epithelial tissues (1,3,6). For example, AE-1/AE-3 is a commonly used specific pan cytokeratin that detects cytokeratins 1-8, 10, 14-16 and 19 (1,3,6).

Given the role of cytokeratins in the structural integrity of epithelial cells, mutations in cytokeratins have been shown to play a role in a variety of human diseases including epidermolysis bullosa simplex (EBS) (4,5). EBS is an autosomal dominant disorder that is caused by missense mutations in either CK5 or CK14 (5). Other known cytokeratin-related disorders include bullous ichthyosis, a skin disorder characterized by redness, blistering, and hyperkeratosis, and epidermolytic palmoplantar keratoderma (EPPK), which results in hyperkeratosis on the palms and soles of the body (7).

References

1. Awasthi, P., Thahriani, A., Bhattacharya, A., Awasthi, P., & Keratins, B. A. (2016). Keratins or cytokeratins: a review article. Journal of Advanced Medical and Dental Sciences Research. https://10.21276/jamdsr.2016.4.4.30

2. Southgate, J., Harnden, P., & Trejdosiewicz, L. K. (1999). Cytokeratin expression patterns in normal and malignant urothelium: a review of the biological and diagnostic implications. Histology and histopathology. https://doi.org/10.14670/HH-14.657

3. Belaldavar, C., Mane, D. R., Hallikerimath, S., Kale, A. D. (2016). Cytokeratins: Its role and expression profile in oral health and disease. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. https://doi.org/10.1016/j.ajoms.2015.08.001.

4. Linder S. (2007). Cytokeratin markers come of age. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1159/000107582

5. Jacob, J. T., Coulombe, P. A., Kwan, R., & Omary, M. B. (2018). Types I and II Keratin Intermediate Filaments. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a018275

6. Ordonez N. G. (2013). Broad-spectrum immunohistochemical epithelial markers: a review. Human pathology. https://doi.org/10.1016/j.humpath.2012.11.016

7. McLean, W. H., & Moore, C. B. (2011). Keratin disorders: from gene to therapy. Human molecular genetics. https://doi.org/10.1093/hmg/ddr379

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-579
Species: Hu, Mu, Bv, Pm
Applications: IHC

Publications for Cytokeratin, pan Antibody (NB600-579)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Monkey.

We have publications tested in 2 applications: IF/IHC, IHC-P.


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IF/IHC
(3)
IHC-P
(3)
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Human
(2)
Mouse
(4)
Monkey
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB600-579 Applications Species
Napso T, Lean SC, Lu M et al. Diet-induced maternal obesity impacts feto-placental growth and induces sex-specific alterations in placental morphology, mitochondrial bioenergetics, dynamics, lipid metabolism and oxidative stress in mice Acta physiologica (Oxford, England) 2022-02-03 [PMID: 35114078] (IF/IHC, Mouse) IF/IHC Mouse
Eber M, Park S, Contino K et al. Osteoblasts derived from mouse mandible enhance tumor growth of prostate cancer more than osteoblasts derived from long bone Journal of Bone Oncology 2021-02-01 [PMID: 33425674] (IHC-P, Mouse) IHC-P Mouse
De Clercq K, Lopez-Tello J, Vriens J, Sferruzzi-Perri A Double-label immunohistochemistry to assess labyrinth structure of the mouse placenta with stereology Placenta 2020-05-01 [PMID: 32421534] (IHC-P, Mouse) IHC-P Mouse
Georges LMC, De Wever O, Galvan JA et al. Cell Line Derived Xenograft Mouse Models Are a Suitable in vivo Model for Studying Tumor Budding in Colorectal Cancer Front Med (Lausanne) 2019-06-27 [PMID: 31316988] (IF/IHC, Human) IF/IHC Human
Slivka, PF;Hsieh, CL;Lipovsky, A;Pratt, SD;Locklear, J;Namovic, MT;McDonald, HA;Wetter, J;Edelmayer, R;Hu, M;Murphy, E;Domanus, M;Lu, C;Duggan, R;King, J;Scott, VE;Donnelly-Roberts, D;Slavin, A;Gopalakrishnan, S;Chung, N;Goedken, ER; Small Molecule and Pooled CRISPR Screens Investigating IL17 Signaling Identify BRD2 as a Novel Contributor to Keratinocyte Inflammatory Responses ACS Chem. Biol. 2019-04-15 [PMID: 30938974] (IHC-P, Human) IHC-P Human
Nicholson SM, Carlesso G, Cheng LI et al. Effects of ICOS+ T cell depletion via afucosylated monoclonal antibody MEDI-570 on pregnant cynomolgus monkeys and the developing offspring Reprod. Toxicol. 2017-09-13 [PMID: 28916434] (Monkey) Monkey
Cuzic S, Bosnar M, Kramaric MD et al. Claudin-3 and Clara Cell 10 kDa Protein as Early Signals of Cigarette Smoke-Induced Epithelial Injury along Alveolar Ducts Toxicol Pathol 2012-12-01 [PMID: 22659244] (IF/IHC, Mouse) IF/IHC Mouse

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FAQs for Cytokeratin, pan Antibody (NB600-579). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any experience staining lymphoepithelium or epithelial cells in general with this antibody? Is this antibody also working on frozen sections of mice specimens?
    • NB600-579 has been validated for use in IHC on frozen and paraffin-embedded tissues in human, mouse and bovine samples. It should be suitable for your experiments.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT