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Cytokeratin, pan Antibody

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Immunohistochemistry-Paraffin: pan Cytokeratin Antibody [NB600-579] - Human skin stained with Cytokeratin.
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody [NB600-579] - Human skin stained with Cytokeratin.

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cytokeratin, pan Antibody Summary

Immunogen
pan Cytokeratin isolated from bovine muzzle epidermis.
Localization
Cytoplasmic
Marker
Epithelial marker
Specificity
NB600-579 has cross-reactivity with pan Cytokeratin of MW 58, 56, 52, 60, 51, 48 and 68 kDa. The antibody is well suited for the staining of a broad spectrum of human keratins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:75-1:200
  • Immunohistochemistry-Frozen 1:75-1:200
  • Immunohistochemistry-Paraffin 1:75-1:200
Application Notes
An incubation time of 30-60 minutes at RT is recommended. Proteolytic enzymatic pretreatment of the tissue sections is also recommended.
Control
Human Skin Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB600-579 in the following applications:

  • IHC
    2 publications
  • IHC-P
    2 publications

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 28916434).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS with BSA
Preservative
Sodium Azide
Purity
Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.
Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, IHC, IHC-P
Cytokeratin 14
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NBP2-34270
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Cytokeratin 5
Antibody (2C2)
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Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.
(1 Review)
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
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Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).
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Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human kidney shows low expression as expected.
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(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
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DNST0
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Immunocytochemistry/Immunofluorescence: OXSM Antibody [NBP1-84730] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: OXSM Antibody [NBP1-84730] - Staining of human cerebral cortex.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 3 Antibody (KRT3/2130).
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Ovary stained with Cytokeratin 3 Antibody (KRT3/2130).
Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 3 Antibody (KRT3/2130). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
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Antibody (SN2001)
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Western Blot: Cytokeratin 4 Antibody (SN2001) [NBP2-67727] - Analysis of Cytokeratin 4 on 293T cells lysates using anti-Cytokeratin 4 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN2001) [NBP2-67727] - Staining Cytokeratin 4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Applications: WB, ICC/IF, IHC, IHC-P
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Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
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Choline Kinase beta [Unconjugated]
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Antibody
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(1 Review)
(4 Publications)
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Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
(36 Publications)
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Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY licence.
Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
(3 Reviews)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
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Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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Immunohistochemistry: KCNN3 Antibody [NBP2-85137] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN3. Gene Name: KCNN3. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School
Western Blot: KCNN3 Antibody [NBP2-85137] - WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
Kir2.1 Antibody
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Western Blot: Kir2.1 Antibody (S112B-14) [NBP2-12900] - analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry: Kir2.1 Antibody (S112B-14) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.
Species: Hu, Mu, Rt, Pm
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Cytokeratin, pan
Antibody
NB600-579
Cytokeratin, pan Antibody
Species: Hu, Mu, Bv, Pm
Applications: IHC, IHC-Fr, IHC-P

Publications for Cytokeratin, pan Antibody (NB600-579)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Monkey.

We have publications tested in 2 applications: IHC, IHC-P.


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IHC
(2)
IHC-P
(2)
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Human
(2)
Mouse
(2)
Monkey
(1)
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FAQs for Cytokeratin, pan Antibody (NB600-579). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any experience staining lymphoepithelium or epithelial cells in general with this antibody? Is this antibody also working on frozen sections of mice specimens?
    • NB600-579 has been validated for use in IHC on frozen and paraffin-embedded tissues in human, mouse and bovine samples. It should be suitable for your experiments.

Control Lysate(s)

Human Skin Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT1
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