Human Skin Whole Tissue Lysate (Adult Whole Normal)

SDS-Page: Human Skin Whole Tissue Lysate (Adult Whole Normal) [NB820-59254] - man Skin Whole Tissue Lysate (Whole Normal) [NB820-59254] - Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.

• Reactivity: Hu
• Applications: WB, IP
• Concentration: 5.0 mg/ml

Human Skin Whole Tissue Lysate (Adult Whole Normal) Summary

• Specificity: Total Protein - Human Adult Normal Tissue: Skin

Applications/Dilutions

• Dilutions:
  • Western Blot 10 ug -20 ug
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Skin
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Skin Lysate (NB820-59254)(2)

Publications using NB820-59254

• Horer S, Marrakchi S, Radner FPW et al. A monoallelic two-hit mechanism in PLCD1 explains the genetic pathogenesis of hereditary trichilemmal cyst formation J. Invest. Dermatol. May 10 2019 [PMID: 31082376] (WB, Human)
• Sherwood AR, Johnson MB, Delgado-Escueta AV, Gentry MS. A bioassay for Lafora disease and laforin glucan phosphatase activity. Clin Biochem. 2013 Sep 6 [PMID: 24012855] (IP)