Cytokeratin, LMW Antibody (AE1) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Other, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, CyTOF-ready
Clone
AE1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Cytokeratin, LMW Antibody (AE1) - Azide and BSA Free Summary

Immunogen
Human epidermal keratin was used as the immunogen for the Cytokeratin LMW AE1 antibody.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI less than 5.7) and basic (pI less than 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT77
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • CyTOF-ready
Application Notes
Enzymatic predigestion of paraffin embedded tissues is recommended prior to the immunostaining.

Reactivity Notes

Turtle

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytokeratin, LMW Antibody (AE1) - Azide and BSA Free

  • Cytokeratin LMW
  • cytokeratin-1B
  • keratin 77
  • keratin, type II cytoskeletal 1b
  • KRT77
  • type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KRT77