Cytokeratin, LMW Antibody (AE-1) [mFluor Violet 500 SE]

Cytokeratin, LMW Antibody (AE-1) [mFluor Violet 500 SE] - Vial of mFluor Violet 500 conjugated antibody. mFluor Violet 500 is optimally excited at 410 nm by the Violet laser (405 nm) and has an emission maximum of 501 nm.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re
• Applications: WB, Flow, ICC/IF, IHC
• Clone: AE-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 500 SE

Cytokeratin, LMW Antibody (AE-1) [mFluor Violet 500 SE] Summary

• Immunogen: Human epidermal keratin (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Turtle

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin, LMW Antibody (AE-1) [mFluor Violet 500 SE]

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT77