Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted [NBP2-48267] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Mouse Monoclonal Antibody (AE-1).

Western Blot Analysis of A431 cells using Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re
• Applications: IHC
• Clone: AE-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Human epidermal keratin (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Turtle

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin, LMW Antibody (AE-1) - IHC-Prediluted

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT77