Cytokeratin, LMW Antibody (AE-1)

Western Blot: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411] - Analysis of A431 lysate using Cytokeratin antibody at 0.25 ug/ml.

Immunohistochemistry-Paraffin: Cytokeratin, LMW Antibody (AE-1) [NBP2-29411] - Formalin-fixed, paraffin-embedded skin stained with Cytokeratin, LMW Ab (AE1)

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re
• Applications: WB, Flow, ICC/IF, IHC
• Clone: AE-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin, LMW Antibody (AE-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33178)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human epidermal keratin (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Turtle

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin, LMW Antibody (AE-1)

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Background

This cytokeratin antibody recognizes most of the acidic (Type I) keratins. seen by western blot as bands between 40 and 56.5 kD. In keratinized epidermis 50 kD keratin is present in the basal layer, while 56.5 kD keratin is present in suprabasal layers. The cytokeratin AE1 antibody can be used to stain distinct cell layers in epithelial tissue and various neoplasms of epithelial origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cytokeratin, LMW Antibody (NBP2-29411). (Showing 1 - 1 of 1 FAQ).

1. Can you tell me what the antigen sequence is for product number NBP2-29411 I guess most importantly I need to know if there is any cross-reactivity with mouse Krt77.
   • NBP2-29411 was immunized against the full length protein. Since we don't epitope map our products, I will not have the specific homology to the mouse sequence, but I can provide you the overall homology. Overall homology is 64%. I would predict that there is a chance that this antibody will recognize the mouse protein. As we have not yet tested this product against mouse samples, I will not be able to say for certain.

Bioinformatics

• Gene Symbol: KRT77
• Uniprot:
  • P04264(Human)
  • Q7Z794(Human)