Cytokeratin, LMW Antibody (AE-1) [HRP]

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re
• Applications: WB, Flow, ICC/IF, IHC
• Clone: AE-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

Cytokeratin, LMW Antibody (AE-1) [HRP] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Human epidermal keratin (Uniprot: Q7Z794)
• Localization: Cytoplasmic
• Marker: Epithelial Marker
• Specificity: This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT77
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Turtle

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for Cytokeratin, LMW Antibody (AE-1) [HRP]

• Cytokeratin LMW
• cytokeratin-1B
• keratin 77
• keratin, type II cytoskeletal 1b
• KRT77
• type-II keratin Kb39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Cytokeratin, LMW Antibody (NBP2-33178H). (Showing 1 - 1 of 1 FAQ).

1. Could you tell us how much the concentration and volume will be?
   • For our directly conjugated products, the product concentration is determined individually for each lot upon completion of the conjugation process. The recovery rate after conjugation is typically 90% or greater. The volume for the conjugate will be tailored to meet the flow test size requirements of 1ul/ 1 x 10^6 cells. So in the case of this product, it will be provided as 100ul units.

Bioinformatics

• Gene Symbol: KRT77