Cytokeratin 8 Antibody (KRT8/899)

Western Blot: Cytokeratin 8 Antibody (KRT8/899) [NBP2-44941] - Western blot shows lysate of Madin-Darby canine kidney epithelial (MDCK GII) cell line. Nitrocellulose membrane was probed with anti-Cytokeratin 8 (Catalog # NBP2-44941) followed by HRP-conjugated secondary antibody. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (KRT8/899) [NBP2-44941] - Human Lung Carcinoma stained with Cytokeratin 8 Monoclonal Antibody (KRT8/899).

Simple Western: Cytokeratin 8 Antibody (KRT8/899) [NBP2-44941] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.2 mg/mL of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 8 Antibody (KRT8/899) [NBP2-44941] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 10 ug/mL dilution of MCF-7 lysates(s) respectively.

Product Discontinued

Cytokeratin 8 Antibody (KRT8/899) Summary

• Immunogen: Recombinant human KRT8 protein
• Localization: Cytoplasmic
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 mL
  • Immunofluorescence 1 - 2 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/mL
  • Simple Western 10 ug/ml
  • Western Blot
• Application Notes: In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
  
  Cytokeratin 8 antibody validated for WB from a verified customer review.
• Theoretical MW: 52.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS, 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 8 Antibody (KRT8/899)

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Background

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 8 Antibody (NBP2-44941)(2)

Publications using NBP2-44941

• Seong J, Frias-Aldeguer J, Holzmann V et al. Iron Overload via Heme Degradation Epiblast inducers capture mouse trophectoderm stem cells in vitro and pattern blastoids for implantation in utero Cell stem cell 2022-07-07 [PMID: 35803228]
• Sozen B, Cox AL, De Jonghe J, et al. Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid Dev. Cell 2019-12-16 [PMID: 31846649]

Review for Cytokeratin 8 Antibody (NBP2-44941) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer WB Canine 08/21/2019

Summary

• Application: Western Blot
• Sample Tested: MDCK canine kidney epithelial cell line
• Species: Canine
• Lot: 3856-9P 180530

Bioinformatics

• Gene Symbol: KRT8