Cytokeratin 8 Antibody (H1) - Azide and BSA Free

Images

 
Western Blot: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 8 Mouse Monoclonal Antibody (H1).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Immunofluorescence Analysis of HCT116 cells labeling CK8 with Cytokeratin 8 Mouse Monoclonal Antibody (H1) ...read more
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 8 MAb (H1).
Flow Cytometry: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 8 Antibody (H1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control ...read more
Western Blot: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Western Blot Analysis of A431 cell lysate using Cytokeratin 8 Mouse Monoclonal Antibody (H1).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Immunofluorescence Analysis of MCF-7 cells labeling CK8 with Cytokeratin 8 Antibody (H1) followed by Goat ...read more
Simple Western: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Simple Western lane view shows a specific band for Cytokeratin 8 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under ...read more
Simple Western: Cytokeratin 8 Antibody (H1) - Azide and BSA Free [NBP2-34626] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 8 antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) ...read more

Product Details

Summary
Reactivity Hu, Rt, ZeSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin 8 Antibody (H1) - Azide and BSA Free Summary

Immunogen
Cytoskeleton preparation containing cytokeratin 8
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Simple Western
  • Western Blot 0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 8 Antibody (H1) - Azide and BSA Free

  • CARD2
  • CK8
  • CK-8
  • CYK8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Bioinformatics

Gene Symbol KRT8