Cytokeratin 6a Antibody - BSA Free

Western Blot: Cytokeratin 6a Antibody [NBP1-31443] - Sample(30 ug of whole cell lysate) A:A431 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody [NBP1-31443] - Mouse cervix. Cytokeratin 6 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Cytokeratin 6a Antibody [NBP1-31443] - D54MG xenograft, using KRT6A antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 6a Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin 6a Antibody - BSA Free (NBP1-31443) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Cytokeratin 6a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 6a. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KRT6A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID: 23858006)
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Cytokeratin 6a Antibody - BSA Free

• Allergen Hom s 5
• CK6A
• CK-6A
• CK6C
• CK6D
• CK-6D
• cytokeratin 6A
• cytokeratin 6C
• cytokeratin 6D
• Cytokeratin-6A
• Cytokeratin-6D
• K6A
• K6C
• K6D
• keratin 6A
• keratin 6C
• keratin 6D
• keratin, epidermal type II, K6A
• keratin, type II cytoskeletal 6A
• Keratin-6A
• KRT6C
• KRT6D
• Type-II keratin Kb6

Background

The human type II Cytokeratin 6 (CK6; 56 kDa) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly the dominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and that the various isoforms are differentially regulated within and between epithelial tissue types (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6, CK6a and MK6b. These genes display a complex expression pattern with constitutive expression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyonychia congenital (PC) is characterized by hypertrophic nail dystrophy and associated ectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16, whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Cytokeratin 6a Antibody (NBP1-31443)(1)

Publication using NBP1-31443

• O'Grady SM, Patil N, Melkamu T et al. ATP release and Ca2+ signaling by Human bronchial epithelial cells following Alternaria aeroallergen exposure. J Physiol 2013-08-05 [PMID: 23858006] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: KRT6A