Human Cytokeratin 5 ELISA Kit (Colorimetric)

• Reactivity: Hu
• Suitable Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Standard Curve Range: 15.6-1000 pg/mL
• Sensitivity: 6.6 pg/mL

Human Cytokeratin 5 ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 15.6-1000 pg/mL
• Sensitivity: 6.6 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Spike Recovery: 93 - 103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KRT5

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Cytokeratin 5 ELISA Kit (Colorimetric)

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KRT5