Cytokeratin 5 Antibody (12C4)

Images

 
Western Blot: Cytokeratin 5 Antibody (12C4) [NBP2-59447] - Lane 1: HeLa cell lysates, Lane 2: A431 cell lysates
Immunocytochemistry/ Immunofluorescence: Cytokeratin 5 Antibody (12C4) [NBP2-59447] - Analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) ...read more
Flow Cytometry: Cytokeratin 5 Antibody (12C4) [NBP2-59447] - Flow cytometry analysis of KRT5 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouse IgG Alexa fluor 488 conjugate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
12C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cytokeratin 5 Antibody (12C4) Summary

Immunogen
Recombinant human KRT5 (1-590aa) purified from E. coli
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT5
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Flow Cytometry 1:10-1:1052
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 5 Antibody (12C4)

  • 58 kDa cytokeratin
  • CK5
  • CK-5
  • cytokeratin-5
  • DDD
  • EBS2
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
  • K5
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
  • keratin 5
  • keratin, type II cytoskeletal 5
  • Keratin-5
  • KRT5A
  • Type-II keratin Kb5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KRT5