Cytokeratin 5/6 Antibody (D5/16B4)

Product Discontinued

Cytokeratin 5/6 Antibody (D5/16B4) Summary

• Immunogen: Human cytokeratin 5.
• Localization: Cytoplasmic
• Specificity: Cytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, intermediate, and superficial cell layers of stratified epithelia. It is also expressed in transitional and complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56kD) is also a high molecular weight basic type cytokeratin expressed by proliferating squamous epithelium that is often paired with cytokeratin 16.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen Neat
  • Immunohistochemistry-Paraffin Neat
• Application Notes: IHC-P: Prediluted and ready to use. We suggest an incubation of 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.IHC-Fr: Prediluted and ready to use on acetone fixed cryostat sections. We suggest an incubation of 30 minutes. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: No buffer
• Preservative: Sodium Azide
• Purity: Protein A or G purified

Notes

This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Alternate Names for Cytokeratin 5/6 Antibody (D5/16B4)

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Background

Cytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with Cytokeratin 16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT5
• Entrez:
  • 3852(Human)